Immune efficacy of carbon nanotubes recombinant subunit vaccine against largemouth bass ulcerative syndrome virus. Issue 100 (May 2020)

• Record Type: Journal Article
• Authors: Jia, Yi-Jun; Guo, Zi-Rao; Ma, Rui; Qiu, De-Kui; Zhao, Zhao; Wang, Gao-Xue; Zhu, Bin
• Is Part Of: Fish & shellfish immunology. Issue 100(2020)
• Page Start: 317
• Page End: 323
• Publication Date: 2020-05
• Subjects: LBUSV -- Largemouth bass -- Single-walled carbon nanotubes -- Subunit vaccine -- Immune response; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2020.03.025
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Largemouth bass ulcerative syndrome virus (LBUSV) is an important virus induce the mortality of largemouth bass ( Micropterus Salmoides ). In this study, we reported a single-walled carbon nanotubes (SWCNTs) containing LBUSV major capsid protein (MCP) subunit vaccine (SWCNTs-MCP) which was evaluated for its protective effect on largemouth bass by immersion immunization. We found an elevation in serum antibody levels, enzyme activities, complement C3 content and immune-related genes (IgM, TGF-β, IL-1β, IL-8, TNF-α and CD4) expression, in the SWCNTs-MCP immunized groups compared with the pure MCP group. The survival rates for control group, pure MCP protein groups (40 mg L −1 ) and four SWCNTs-MCP groups (5 mg L −1, 10 mg L −1, 20 mg L −1 and 40 mg L −1 ) were 0%, 27.78%, 30.56%, 50.00%, 66.67% and 80.56%, respectively. The results suggests that with the assistance of SWCNTs, the immune protection of the SWCNTs-MCP group (40 mg L −1 ) increased up 52.78%–80.1% compared with pure MCP group (40 mg L −1 ). Our results demonstrate that the single-walled carbon nanotube subunit vaccine can be used as a new immunization method against LBUSV showing protection following challenge with LBUSV. Taken together, our results demonstrate that the single-walled carbon nanotube subunit vaccine can be used as a new method against LBUSV and have a high immune protection during the largemouth bass farm. Highlights: Largemouth bass immersed with SWCNTs-MCP confers strong immunity against LBUSV. SWCNTs enhance the immunological effect of bath vaccines. SWCNTs based immersion vaccine have prospects for application in aquatic vaccine.