Β-D-glucan from marine yeast Debaryomyces hansenii BCS004 enhanced intestinal health and glucan-expressed receptor genes in Pacific red snapper Lutjanus peru. (June 2020)

• Record Type: Journal Article
• Authors: Reyes-Becerril, Martha; Angulo, Miriam; Sanchez, Veronica; Guluarte, Crystal; Angulo, Carlos
• Is Part Of: Microbial pathogenesis. Volume 143(2020)
• Publication Date: 2020-06
• Subjects: Functional carbohydrates -- Antioxidant activity -- Fish -- Intestine; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2020.104141
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: Previous studies have shown that marine yeast Debaryomyces hansenii BCS004 (also known as Dh004) has a potential biotechnological application. The aim of this study was to investigate the structural characterization, antioxidant properties and possible health inductor of dietary β-D-glucan BCS004. In this study, a glucan BCS004 was obtained containing (1–6)-branched (1–3)-β-D-glucan with low molecular weight and a high purity of 90 and 91.7% for one and 4 h, respectively. β-D-glucan BCS004 showed higher antioxidant activity, including DPPH radical and superoxide anion scavenging, β-carotene bleaching inhibition, and iron chelation activity. An in vitro study showed that β-D-glucan BCS004 was safe for peripheral blood leukocytes inducing proliferative effects. Moreover, in an in vivo study using β-D-glucan BCS004 no histopathological damages or intestinal inflammation were observed in fish. The gene expression analysis highlighted that dietary β-D-glucan BCS004 could also up-regulate glucan and macrophage receptor genes in intestine, such as C-type lectin (CTL) and macrophage mannose receptors (MMR). Overall, the results demonstrated that β-D-glucan from D. hansenii BCS004 could be an immunostimulant with antioxidant properties and beneficial effects on intestinal health in fish. Highlights: Glucan from marine yeast Debaryomyces hansenii BCS004 (Dh004) was characterized. NMR indicated structures of (1–6)-branched (1–3)-β-D-glucan with low molecular weight. β-D-glucan BCS004 exhibited significant free radical scavenging capacity. β-D-glucan BCS004 is not cytotoxic for peripheral blood leukocytes and intestine. β-D-glucan BCS004 up-regulated glucan and cellular receptor genes in intestine.