Dual detection of biochemical oxygen demand and nitrate in water based on bidirectional Shewanella loihica electron transfer. (August 2020)

• Record Type: Journal Article
• Authors: Yi, Yue; Zhao, Ting; Xie, Beizhen; Zang, Yuxuan; Liu, Hong
• Is Part Of: Bioresource technology. Volume 309(2020)
• Publication Date: 2020-08
• Subjects: BOD detection -- Nitrate detection -- Biosensor -- Bioelectrochemical system -- Bidirectional extracellular electron transfer; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2020.123402
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Establishes the relationship between the inward current of BES and nitrate. Reveals a new characteristic of ano-cathodophilic capability of S. loihica biofilm. Reports the first BES sensor for simultaneous detection of BOD and nitrate in water. Achieves a high accuracy of >80% and fast analysis within 1 h. Expands the application of bidirectional EET in water monitoring. Abstract: This study for the first time proposed a method for simultaneously measuring BOD and nitrate in water using electrochemically active bacteria. Firstly, the bidirectional extracellular electron transfer (EET) capability of a model electricigen Shewanella loihica PV-4 was revealed. Then, based on the respective outward and inward EET, S. loihica PV-4 was utilized to detection BOD and nitrate. The results demonstrated a positive correlation between the outward EET and BOD (from 0 mg/L to 435 mg/L) while a negative correlation between the inward EET and nitrate (from 0 mg/L to 7 mg/L); both the relationships were well fitted by the combination of traditional linear model and Michaelis-Menten model (R 2 > 0.96). Finally, a dual detection method for BOD and nitrate measurements was established based on the ano-cathodophilic capability of S. loihica PV-4 biofilm, and exhibited the characteristics of high accuracy (>80%) and fast analysis (<1 h), suggesting a promising prospect in water monitoring.