Ganoderma atrum polysaccharide ameliorates intestinal mucosal dysfunction associated with autophagy in immunosuppressed mice. (April 2020)

• Record Type: Journal Article
• Authors: Ying, Mengxi; Zheng, Bing; Yu, Qiang; Hou, Kunyou; Wang, Hui; Zhao, Mingming; Chen, Yi; Xie, Jianhua; Nie, Shaoping; Xie, Mingyong
• Is Part Of: Food and chemical toxicology. Volume 138(2020)
• Publication Date: 2020-04
• Subjects: Ganoderma atrum polysaccharide -- Intestinal immunity -- Mucosal integrity -- Autophagy -- Cyclophosphamide; Toxicology -- Periodicals; Food poisoning -- Periodicals; Food Poisoning -- Periodicals; Toxicology -- Periodicals; Toxicologie -- Périodiques; Intoxications alimentaires -- Périodiques; Food poisoning; Toxicology; Periodicals; Electronic journals; 615.9
• DOI: 10.1016/j.fct.2020.111244
• Languages: English
• ISSNs: 0278-6915

Abstract

Abstract: The aim of this study was to investigate the protective effect and underlying mechanisms of Ganoderma atrum polysaccharide (PSG-1) on cyclophosphamide (Cy)-induced intestinal mucosal dysfunction in mice. Results showed that PSG-1 promoted the formation of IgA-secreting cells, modulated sIgA, IgE, IgG, IgM secretion, and improved TLR-2, TLR-4, TLR-6 mRNA levels while these factors were suppressed after Cy treatment. CD4 + and CD8 + T cell numbers were also elevated by PSG-1. Cytokines including IFN-γ, TNF-α, IL-2, IL-12p70, IL-4, IL-1β, IL-17, IL-21, IL-23, TGF-β3 and transcription factors including T-bet, GATA-3, RORγt, Foxp3 increased after PSG-1 administration. Besides, PSG-1 reversed goblet cell numbers, and upregulated tight junction proteins like ZO-1, occludin and claudin-1 in immunosuppressed mice. Apart from these, the autophagy-related proteins LC3, Beclin-1, Atg5 and Atg7 were enhanced by PSG-1. These findings demonstrated that PSG-1 could ameliorate Cy-induced impairment of intestinal immunity and mucosal integrity, which maybe associated with autophagy in mice. Graphical abstract: Image 1 Highlights: PSG-1 promoted IgA-secreting cells formation, modulated Igs secretion, and improved TLRs levels. PSG-1 elevated CD4 + and CD8 + T cell numbers in cyclophosphamide (Cy)-treated mice. PSG-1 regulated cytokine environment and transcription factors production in Cy-treated mice. PSG-1 increased goblet cell numbers and upregulated tight junction proteins in Cy-treated mice. PSG-1 strengthened autophagy via regulating autophagy-related proteins in Cy-treated mice.