Sandalwood-derived carbon quantum dots as bioimaging tools to investigate the toxicological effects of malachite green in model organisms. (June 2020)

• Record Type: Journal Article
• Authors: Shukla, Devyani; Das, Megha; Kasade, Dipanshu; Pandey, Maneesha; Dubey, Ashutosh Kumar; Yadav, Sanjeev Kumar; Parmar, Avanish Singh
• Is Part Of: Chemosphere. Volume 248(2020)
• Publication Date: 2020-06
• Subjects: CQD -- MG -- Onion -- Mung -- Fluorescence spectroscopy -- Bioimaging -- Santalum album; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2020.125998
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: Malachite green is an N-methylated diaminophenylmethane dye that has generated much concern over its suggestive carcinogenic nature. After its excessive use in aquaculture industry as an effective ectoparasitide, much debate was raised over its toxicological effects leading to scientific studies conducted on animal models. Even after several bans, malachite green is still easily available in many parts of the world and unscrupulously even used to give green vegetables a fresher look. This study aims to address this concern by systematically studying the toxicological effects of malachite green through bioimaging in plant and animal cell and tissue. Sandalwood-derived carbon quantum dots have been used as a bioimaging tool since they are non-cytotoxic and show excellent fluorescence properties. Onion tissues demonstrate the translocation of the dye inside cells having high affinity for the nuclei and cell walls. Toxicological effects on the growth of Vigna radiata (mung beans) have been studied methodically. Bioimaging of the transverse cross-section of the dye-treated plant root shows a significant difference from the control. In animal cells, dose-dependent decrease in cell viability of MG-63 cells was observed with MG. CQD showed good fluorescence in both cytoplasm and nucleus of MG63 cells. In addition, CQDs were employed as a great tool for bioimaging of the histopathologically adverse effects of MG in Golden hamster animal model. This study showed CQDs could be used as an alternative non-site specific fluorescent probe for cell and tissue imaging for better visualization of cell and tissue architectural changes. Graphical abstract: Schematic Illustrating the Bioimaging Studies Conducted on various plant ( Allium cepa, Vigna radiata ) and animal model systems (MG-63 osteoblast cell line, golden hamster) for investigating the toxicological effects of malachite green using sandalwood-derived carbon quantum dots as fluorescence probes. Image 1 Highlights: Toxicity study of malachite green using carbon dots in onion cells and Vigna radiata showed dye localization and adverse growth effects. LCLO at which mung seeds refuse to germinate was found to be 11 mM. IC50 in MG-63 cells was found to be 4.559 μM. LD50 for golden hamster found to be 27 mg/kg body weight. Carbon quantum dot-assisted luorescence images of malachite green treated golden hamster tissues show severe atrophy.