Multifunctional properties of spherical silver nanoparticles fabricated by different microbial taxa. Issue 5 (May 2020)

• Record Type: Journal Article
• Authors: Alsharif, Sultan M.; Salem, Salem S.; Abdel-Rahman, Mohamed Ali; Fouda, Amr; Eid, Ahmed Mohamed; El-Din Hassan, Saad; Awad, Mohamed A.; Mohamed, Asem A.
• Is Part Of: Heliyon. Volume 6:Issue 5(2020)
• Publication Date: 2020-05
• Subjects: Materials science -- Nanotechnology -- Agriculture -- Spherical silver nanoparticles -- Ag-NPs biosynthesis -- Antibacterial -- Antitumor activities -- Larvicidal -- Aedes aegypti; Research -- Periodicals; Medical sciences -- Periodicals; Natural history -- Periodicals; Social sciences -- Periodicals; Earth sciences -- Periodicals; Physical sciences -- Periodicals; 507.2
• DOI: 10.1016/j.heliyon.2020.e03943
• Languages: English
• ISSNs: 2405-8440

Abstract

Abstract: This study addresses the impacts of metabolites from different microbial taxa on the fabrication and multifunctional biological properties of spherical silver nanoparticles (Ag-NPs). Three microbial taxa, a bacterial ( Bacillus cereus A1-5), actinomycetes ( Streptomyces noursei H1-1), and fungal ( Rhizopus stolonifer A6-2) strains were used for Ag-NPs biosynthesis, whereas Streptomyces noursei is demonstrated for the first time. These isolates were identified using either 16S rRNA or ITS gene sequencing. Characterization of Ag-NPs was done using color change analysis, Uv-Vis spectroscopy, FT-IR spectroscopy, XRD, TEM, SEM-EDX, DLS, and Zeta potential analysis. All biosynthesized NPs exhibited spherical shape with different sizes ranged from 6‒50 nm, 6–30 nm and 6–40 nm for NPs obtained by A1-5, H1-1 and A6-2, respectively. The crystalline center cubic face of Ag-NPs was confirmed using XRD at 2 θ values 38.08 o, 44.27 o, 64.41 o and 77.36 o . FT-IR analysis revealed varied intense absorption peaks for biomolecules required for NPs synthesize by each microbial strain. The stability of spherical Ag-NPs was confirmed due to highly DLS negative surface charge of ‒17.5mV, ‒18.9mV, and ‒15.6mV for NPs synthesized by strains A1-5, H1-1, and A6-2, respectively. Ag-NPs exhibited a broadspectrum of antibacterial activity against Gram-positive and Gram-negative bacteria with varied effectiveness. They also exhibited a cytotoxic effect against cancer cell line (caco-2) in a dose-dependent pattern with IC50 of 8.9 ± 0.5, 5.6 ± 3.0, 11.2 ± 0.5 μg/ml for NPs synthesized by strains A1-5, H1-1, and A6-2, respectively. Moreover, these spherical Ag-NPs showed larvicidal activity against the 3 rd instar larvae of the dengue vector Aedes aegypti . Abstract : Materials Science; Nanotechnology; Agriculture; Spherical silver nanoparticles; Ag-NPs biosynthesis; Antibacterial; Antitumor activities; Larvicidal; Aedes aegypti