Nitrate removal characteristics and 13C metabolic pathways of aerobic denitrifying bacterium Paracoccus denitrificans Z195. (July 2020)

• Record Type: Journal Article
• Authors: Zhang, Haihan; Li, Sulin; Ma, Ben; Huang, Tinglin; Qiu, Hui; Zhao, Zhenfang; Huang, Xin; Liu, Kaiwen
• Is Part Of: Bioresource technology. Volume 307(2020)
• Publication Date: 2020-07
• Subjects: G6P glucose-6-phosphate -- F6P fructose-6-phosphate -- FBP fructose 1, 6-bisphosphate -- 6PG 6-phosphogluconate -- Ru5P ribulose-5-phosphate -- R5P ribose-5-phosphate -- GAP glyceraldehyde 3-phosphate -- 3PG 3-phoshoglycetate -- PEP phosphoenolpyruvate -- PYR pyruvate -- Ace CoA Acetyl-CoA -- OAA oxaloacetate -- CIT citrate -- ICIT isocitrate -- AKG α-ketoglutarate -- SUC succinate -- FUM fumarate -- GLX glyoxylate -- MAL malate -- NADH nicotinamide adenine dinucleotide; Aerobic denitrifying -- Paracoccus denitrificans -- Stable isotopic labeling -- Nitrate removal; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2020.123230
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphic abstract: Highlights: Strain Z195 was identified as Paracoccus denitrificans. Removal efficiencies of NO3 – -N, TN and TOC were 98%, 94% and 98%. Entner-Doudoroff pathway and TCA cycle was revealed by 13 C- MFA. The RSM model for optimal TN removal conditions was established. TN and COD removal efficiencies of raw wastewater were 89% and 91%. Abstract: Strain Z195 was isolated and identified as Paracoccus denitrificans . Z195 exhibited efficient aerobic denitrification and carbon removal abilities, and removed 93.74% of total nitrogen (TN) and 97.81% of total organic carbon.71.88% of nitrogen was lost as gaseous products. 13 C-metabolic flux analysis revealed that 95% and 132% of the carbon fluxes entered the Entner-Doudoroff (ED) pathway and tricarboxylic acid (TCA) cycle, respectively. Electrons produced by carbon metabolism markedly promoted the processes of nitrogen metabolism process and aerobic respiration. A response surface methodology model demonstrated that the optimal conditions for the maximum TN removal were a C/N ratio of 7.47, shaking speed of 108 rpm, temperature of 31 °C and initial pH of 8.02. Additionally, the average TN and chemical oxygen demand removal efficiencies of raw wastewater were 89% and 91%, respectively. The results give new insight for understanding metabolic flux analysis of aerobic denitrifying bacteria.