A novel Adiponectin receptor (AdipoR) involved in regulating cytokines production and apoptosis of haemocytes in oyster Crassostrea gigas. (September 2020)

• Record Type: Journal Article
• Authors: Ge, Wenjing; Huang, Shu; Liu, Shujing; Sun, Jiejie; Liu, Zhaoqun; Yang, Wenwen; Wang, Lingling; Song, Linsheng
• Is Part Of: Developmental and comparative immunology. Volume 110(2020)
• Publication Date: 2020-09
• Subjects: Crassostrea gigas -- APN -- CgAdipoR -- Immunomodulation -- Haemocyte; Immunology -- Periodicals; Developmental immunology -- Periodicals; 616.079
• DOI: 10.1016/j.dci.2020.103727
• Languages: English
• ISSNs: 0145-305X

Abstract

Abstract: Adiponectin receptors (AdipoRs) comprise a seven-transmembrane domain-containing protein family, which specifically recognize adiponectin (APN) and play critical roles in the immunological and physiological processes in vertebrates. In the present study, a novel AdipoR is identified from oyster Crassostrea gigas (designated as Cg AdipoR). The full-length cDNA of Cg AdipoR is of 1209 bp encoding a polypeptide of 343 amino acids. There is an N-terminal domain, a Hly III domain, and a C-terminal domain in Cg AdipoR. After the transfection of Cg AdipoR, the level of intracellular Ca 2+ into HEK293T cells increases significantly (1.36-fold, p < 0.05) after APN incubation. The mRNA transcripts of Cg AdipoR are widely distributed in all the tested tissues, with the highest expression level in haemocytes (3.20-fold of that in hepatopancreas, p < 0.05). After lipopolysaccharide (LPS), Vibrio splendidus and polyinosinic-polycytidylic acid (poly (I:C)) stimulations, the mRNA expression of Cg AdipoR in haemocytes is significantly up-regulated and reached the highest level at 24 h (15.07-fold, p < 0.01), 6 h (4.39-fold, p < 0.01) and 24 h (5.62-fold, p < 0.01) compared to control group, respectively. After Cg AdipoR is interfered by specific Cg AdipoR-dsRNA, the expression level of interleukins ( Cg IL17-1, Cg IL17-2, Cg IL17-3 and Cg IL17-5) in haemocytes decreases significantly ( p < 0.01) at 24 h post LPS stimulation, while the expression level of Cg TNF-1 increases significantly (1.68-fold, p < 0.01), compared to that in the dsEGFP group. In Cg AdipoR dsRNA-injected oysters, the mRNA expressions of anti-apoptotic B-cell lymphoma-2 (Bcl-2) in haemocytes significantly decreases at 24 h after LPS challenge, which is (0.58-fold, p < 0.05) of that in dsEGFP-injected oysters, while the apoptotic rate of haemocytes is significantly up-regulated (1.93-fold of that in dsEGFP group, p < 0.05). These results collectively suggest that Cg AdipoR plays an important role in the immune response of oysters by regulating the expressions of inflammatory cytokines and haemocyte apoptosis. Highlights: Cg AdipoR was identified from oyster, which was constitutively expressed in hemocytes. The expression of Cg AdipoR in the hemocytes was significantly up-regulated after LPS stimulation. After Cg AdipoR was knockdown, the expressions of some Cg IL17s, were inhibited, while that of Cg TNF-1 increased. Cg AdipoR regulated apoptosis of hemocyte during immune response in oyster.