Boost carbon availability and value in algal cell for economic deployment of biomass. (March 2020)

• Record Type: Journal Article
• Authors: Sun, Han; Li, Xiaojie; Ren, Yuanyuan; Zhang, Huaiyuan; Mao, Xuemei; Lao, Yongmin; Wang, Xia; Chen, Feng
• Is Part Of: Bioresource technology. Volume 300(2020)
• Publication Date: 2020-03
• Subjects: Central carbon metabolism -- Carbon availability -- Path rate -- Biomass value -- Microalgae; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2019.122640
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: Carbon efficiency is defined as carbon availability and biomass value. Directly transiting glucose into carbohydrate results in the highest μmax . Converting main carbon sink increases 38.30% astaxanthin productivities. Carbon behaviors can be predicted for the production with 30% increased revenue. Cultivation factors are linked to market scenario for the first time. Abstract: This study elucidated storage carbon metabolism in a dynamic manner through kinetic model, metabolomics and stable metabolic flux analysis. Results revealed nutrient uptake rate, carbon availability and synthetic path rate accounted for the integration of process-compatible products. The uptake rate could be enhanced by promoting carbohydrate accumulation, inducing high performance of tricarboxylic acid cycle and anaplerotic routes. Values of specific rate for lipid from kinetic model and synthetic path rate from metabolic flux analysis revealed that conversion of carbon sinks occupied a key position in increasing productivities of lipid and astaxanthin to 302.34 and 1.83 mg g −1 d −1, respectively. Additionally, economic estimation was applied to link cultivation factors with market scenario and demonstrated that regulating such carbon metabolism raised 30% increase of biomass value. This study therefore provided a new orientation to boost carbon efficiency that helped to engineer carbon flux from carbon source to targeted products precisely and rapidly.