Internalization and antigen presentation by mouse dendritic cells of rotavirus VP6 preparations differing in nanostructure. (July 2020)

• Record Type: Journal Article
• Authors: Tamminen, Kirsi; Heinimäki, Suvi; Gröhn, Stina; Blazevic, Vesna
• Is Part Of: Molecular immunology. Volume 123(2020:Jul.)
• Page Start: 26
• Page End: 31
• Publication Date: 2020-07
• Subjects: APC antigen presenting cell -- VLP virus-like particle -- RV rotavirus -- T-VP6 tubular VP6 nanostructure -- S-VP6 spherical VP6 nanostructure -- nonTS-VP6 non-tubular/non-spherical VP6 assembly -- BMDC bone-marrow dendritic cell -- NoV norovirus -- MHC major histocompatibility complex -- TEM transmission electron microscope -- PdI polydispersity index -- DLS dynamic light-scattering -- GM-CSF granulocyte-macrophage colony-stimulating factor -- FITC fluorescein isothiocyanate -- PE phycoerythrin -- FSC forward scatter -- SSC side scatter -- ELISPOT enzyme-linked immunosorbent spot -- IFN-γ interferon gamma -- SFC spot forming cells -- OPD o-phenylenediamine dihydrochloride -- OD optical density; Rotavirus -- VP6 -- Vaccine -- Platform -- Nanoparticle -- Dendritic cell; Immunochemistry -- Periodicals; Molecular biology -- Periodicals; Immunochemistry -- Periodicals; Allergy and Immunology -- Periodicals; Molecular Biology -- Periodicals; Immunochimie -- Périodiques; Biologie moléculaire -- Périodiques; Immunochemistry; Molecular biology; Periodicals; Electronic journals; 571.96
• DOI: 10.1016/j.molimm.2020.04.001
• Languages: English
• ISSNs: 0161-5890

Abstract

Abstract: Nanoparticles are highly immunogenic due to the multivalent, repetitive antigen expression and direct activation of antigen presenting cells (APCs), key players of adaptive immune responses. Different virus-like particles (VLPs) have been used as display platforms to amplify immune responses to biologically relevant, but poorly immunogenic foreign antigens. A candidate platform based on rotavirus (RV) inner-capsid protein VP6 oligomers, such as nanotubes (T-VP6) and nanospheres (S-VP6), is also considered. Different VP6 nanostructures were compared for internalization and antigen presentation by the APCs. We found, that a lack of a high-order structures, T-VP6 and S-VP6, did not negatively affect VP6 uptake and presentation by murine bone-marrow derived dendritic cells (BMDCs) in vitro . Furthermore, T-VP6 was preferable to norovirus (NoV) VLPs for BMDC internalization resulting in significantly higher VP6-specific immune responses when T-VP6 and NoV VLP pulsed BMDCs were transferred to syngeneic mice. These results support the use of different VP6 nanostructures as foreign antigen delivery platforms.