Exploring fermentation strategies for enhanced lactic acid production with polyvinyl alcohol-immobilized Lactobacillus plantarum 23 using microalgae as feedstock. (July 2020)

• Record Type: Journal Article
• Authors: Chen, Po-Ting; Hong, Zih-Syuan; Cheng, Chieh-Lun; Ng, I-Son; Lo, Yung-Chung; Nagarajan, Dillirani; Chang, Jo-Shu
• Is Part Of: Bioresource technology. Volume 308(2020)
• Publication Date: 2020-07
• Subjects: Lactobacillus plantarum -- Polyvinyl alcohol -- Lactic acid -- Immobilized cells -- Fermentation strategy -- Microalgae; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2020.123266
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: Lactic acid (LA) fermentation allows green production of lactic acid. PVA-immobilization of L. plantarum enhanced LA titer and yield by 40%. Continuous fermentation with glucose gave the highest productivity of 14.2 g/L/h. In situ lactic acid removal during fermentation improved LA productivity & yield. LA productivity reached 9.93 g/L/h when using microalgal hydrolysate as substrate. Abstract: Lactic acid (LA) fermentation was conducted with suspended and immobilized cells of an isolated Lactobacillus plantarum 23 strain using various fermentation strategies. Glucose and an alternative, relatively inexpensive carbon source - the hydrolysate of microalga Chlorella vulgaris ESP-31, were used as the carbon source. Batch fermentation using immobilized cells of L. plantarum 23 could enhance LA titer and yield by 43% and 39%, respectively, when compared with the suspended culture. Fed-batch culture integrated with in situ LA removal via ion exchange raised LA productivity by 72% by overcoming product inhibition. The highest LA productivity from glucose with PVA immobilized cells was 14.22 g/L/h, achieved under continuous operation at 50% w/v loading of immobilized beads and hydraulic retention time (HRT) of 2 h. PVA immobilized L. plantarum 23 could also use microalgal hydrolysate as the renewable carbon source, and the highest LA productivity was 9.93 g/L/h under continuous fermentation at 4 h HRT.