Biomimicry of microbial polysaccharide hydrogels for tissue engineering and regenerative medicine – A review. (1st August 2020)

• Record Type: Journal Article
• Authors: Ng, Jian Yao; Obuobi, Sybil; Chua, Mei Ling; Zhang, Chi; Hong, Shiqi; Kumar, Yogesh; Gokhale, Rajeev; Ee, Pui Lai Rachel
• Is Part Of: Carbohydrate polymers. Volume 241(2020)
• Publication Date: 2020-08-01
• Subjects: Alpha-tricalcium phosphate (PubChem CID: 223738661) -- Chitosan (PubChem CID: 71853) -- Halloysite nanotubes (PubChem CID: 329760969) -- Konjac (PubChem CID: 404772408) -- Magnetite (PubChem CID: 176330884) -- Manuka honey (PubChem CID: 381129233) -- Mesoporous silica (PubChem CID: 329769031) -- Polypyrrole (PubChem CID: 386264466) -- Polyvinyl alcohol (PubChem CID: 11199) -- Sanguinarine (PubChem CID: 5154); Microbial polysaccharide hydrogel -- Tissue engineering and regenerative medicine (TERM) -- Biofunctionalization -- Material blending -- Cell proliferation; 3D three dimension -- μCT microcomputed tomography -- ACC amorphous calcium carbonate -- ADSC adipose-derived stem cell -- AF annulus fibrous -- ALP alkaline phosphatase -- ATCC American Type Culture Collection -- BAG bioactive glass -- BMSC bone marrow stromal cell -- CA carbonic anhydrase -- CaCO3 calcium carbonate -- CaCl2 calcium chloride -- CaGP calcium glycerophosphate -- CaP calcium phosphate -- CD44 cluster of differentiation 44 -- CECS N-carboxyethyl chitosan -- CLSM confocal laser scanning microscopy -- CMC carboxymethyl cellulose -- CPUN cationic polyurethane soft nanoparticles -- DBP demineralized bone powder -- DexS dextran sulfate -- DMSO dimethyl sulfoxide -- DNA deoxyribonucleotide acid -- EAC Ehrlich ascites carcinoma -- ECM extracellular matrix -- FDA food and drug administration -- GAGs glycosaminoglycans -- GD gallus var domesticus -- GG-PEGDA gellan gum-poly(ethylene glycol) diacrylate -- GGMA methacrylated gellan gum -- HA hyaluronan -- HAp hydroxyapatite -- HDF human dermal fibroblast -- HNT halloysite nanotubes -- hMSC human mesenchymal stem cells -- HNSC human neural stem cell -- HUVEC human umbilical vein endothelial cell -- ICP-OES inductively coupled plasma optical emission spectrometry -- ISH ion-sensitive hydrogel -- KCl potassium chloride -- LDH lactate dehydrogenase -- MTT ((3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide)) -- MTS ((3-(4, 5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium)) -- MRSA Methicillin-resistant Staphylococcus aureus -- MSC mesenchymal stem cell -- NCH nanocomposite hydrogel -- NP nucleus pulposus -- OC ostechondral -- PBS phosphate buffered saline -- PCL polycaprolactone -- PDMS polydimethylsiloxane -- PEI polyethyleneimine -- PET positron emission tomography -- PLA (poly(lactic acid)) -- PPy polypyrrole -- PVA polyvinyl alcohol -- qPCR quantitative polymerase chain reaction -- rGO reduced graphene oxide -- ROS reactive oxygen species -- RT-PCR reverse transcription-polymerase chain reaction -- SEM scanning electron microscopy -- SF/GG silk fibroin/gellan gum -- TCP alpha-tricalcium phosphate -- TERM tissue engineering and regenerative medicine -- Tg–s sol-gel transition temperature -- Tgelation gelation temperature -- TGG thiolated gellan gum -- TiO2 titanium oxide -- TNF-α tumor necrosis factor alpha -- U urease -- XG xanthan gum; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2020.116345
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: Biomimicry of microbial polysaccharide hydrogels as TERM scaffolds is discussed. Microbial polysaccharides form hydrogels easily but lack bioactivity. Emphasis is placed on material blending with bioactive materials for bioactivity. A list of organic and inorganic bioactive materials is curated in this review. Abstract: Hydrogels as artificial biomaterial scaffolds offer a much favoured 3D microenvironment for tissue engineering and regenerative medicine (TERM). Towards biomimicry of the native ECM, polysaccharides from Nature have been proposed as ideal surrogates given their biocompatibility. In particular, derivatives from microbial sources have emerged as economical and sustainable biomaterials due to their fast and high yielding production procedures. Despite these merits, microbial polysaccharides do not interact biologically with human tissues, a critical limitation hampering their translation into paradigmatic scaffolds for in vitro 3D cell culture. To overcome this, chemical and biological functionalization of polysaccharide scaffolds have been explored extensively. This review outlines the most recent strategies in the preparation of biofunctionalized gellan gum, xanthan gum and dextran hydrogels fabricated exclusively via material blending. Using inorganic or organic materials, we discuss the impact of these approaches on cell adhesion, proliferation and viability of anchorage-dependent cells for various TERM applications.'