Effect of interpolymer complex formation between chondroitin sulfate and chitosan-gelatin hydrogel on physico-chemical and rheological properties. (15th June 2020)

• Record Type: Journal Article
• Authors: S, Ponsubha; Jaiswal, Amit Kumar
• Is Part Of: Carbohydrate polymers. Volume 238(2020)
• Publication Date: 2020-06-15
• Subjects: β-GP glycerol phosphate disodium salt -- SHC sodium hydrogen carbonate -- C chitosan -- GE gelatin -- CS chondroitin sulfate A salt -- BSA bovine serum albumin -- VEGF165 vascular endothelial growth factor A -- FBS fetal bovine serum -- DMEM dulbecco's modified eagle medium -- MTT methylthiazolyl diphenyl- tetrazolium bromide -- DMSO dimethyl sulfoxide -- HWJ MSC human wharton's jelly mesenchymal stem cells; Chitosan -- Hydrogel -- Chondroitin sulfate -- Glycerol phosphate disodium salt -- Sodium hydrogen carbonate -- Physio-chemical -- Self-healing -- Rheology; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2020.116179
• Languages: English
• ISSNs: 0144-8617

Abstract

Graphical abstract: Highlights: Chondroitin sulfate incorporated chitosan-gelatin hydrogels were prepared using β-glycerol phosphate and sodium hydrogen carbonate. Addition of chondroitin sulfate enhanced the viscoelastic behavior of the hydrogels. The structural breakdown not observed in time sweep experiment depicted the solid-like nature of hydrogel. Protein adsorption seemed to improve in the presence of chondroitin sulfate. HWJ-MSC viability showed efficient biocompatibility of hydrogel. Abstract: The present study focuses on the fabrication of three-dimensional (3D) natural polymeric hydrogel containing chitosan (C), gelatin (GE) and chondroitin sulfate A (CS) to surpass the drawbacks of chitosan-gelatin (C-GE) hydrogel. Hydrogels were prepared using dual gelling agents in the form of β-glycerol phosphate (β-GP) and sodium hydrogen carbonate (SHC) and failed to gel in the absence of either gelling agent. All the hydrogels showed the ability to self-heal when broken into two parts. The addition of CS resulted in the formation of elastic hydrogels during the entire range of applied shear strain (0.001–100 %) compared to hydrogel without CS. CS containing hydrogels resulted in 25–41 % bovine serum albumin in vitro release compared to 77 % in hydrogel without CS at the end of 16 days. The addition of VEGF165 to hydrogel improved cell proliferation drastically in C-GE hydrogel. C-GE-CS hydrogels exhibited better cell growth compared to C-GE hydrogel with or without the addition of VEGF165.