Study on the virulome and resistome of a vancomycin intermediate-resistance Staphylococcus aureus. (August 2020)

• Record Type: Journal Article
• Authors: Fu, Jie; Wang, Kan; Ye, Congxiu; Chen, Ling; Liang, Yi; Mao, Yuzhu; Chen, Jinxuan; Peng, Ruixin; Chen, Yanni; Shi, Fan; Huang, Teng-Yi; Liu, Junyan
• Is Part Of: Microbial pathogenesis. Volume 145(2020)
• Publication Date: 2020-08
• Subjects: Vancomycin intermediate-resistance -- Staphylococcus aureus -- Genomic features -- Virulome -- Resistome; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2020.104187
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: Methicillin-resistant S. aureus (MRSA) has been considered a potential "Super Bugs", responsible for various infectious diseases. Vancomycin has been the most effective antibitic to treat MRSA originated infections. In this study, we aimed at investigating the genomic features of a vancomycin intermediate-resistance S. aureus strain Guangzhou-SauVS2 isolated from a female patient suffering from chronic renal function failure, emphasizing on its antimicrobial resistance and virulence determinants. The genome has a total length of 2, 605, 384 bp and the G+C content of 33.21%, with 2, 239 predicted genes annotated with GO terms, COG categories, and KEGG pathways. Besides the carriage of vancomycin b-type resistance protein responsible for the vancomycin intermediate-resistance, S. aureus strain Guangzhou-SauVS2 showed resistance to β-lactams, quinolones, macrolide, and tetracycline, due to the acquisition of corresponding antimicrobial resistance genes. In addition, virulence factors including adherence, antiphagocytosis, iron uptake, and toxin were determined, indicating the pathogenesis of the strain. Highlights: The carriage of vancomycin b-type resistance protein may contribute to the vancomycin intermediate-resistance. β-lactam, quinolone, macrolide and tetracycline resistance may due to corresponding antibiotic resistance genes carriage. The genome has a total length of 7, 816, 153 bp and the G+C content of 34.27%, with 6, 697 predicted genes. Virulence factors were determined, indicating the pathogenesis of the strain.