Distinct microbial communities and their networks in an anammox coupled with sulfur autotrophic/mixotrophic denitrification system. (July 2020)

• Record Type: Journal Article
• Authors: Du, Shuai; Ya, Tao; Zhang, Minglu; Zhu, Minghan; Li, Nankun; Liu, Shuwei; Wang, Xiaohui
• Is Part Of: Environmental pollution. Volume 262(2020)
• Publication Date: 2020-07
• Subjects: Anammox -- Sulfur-supported denitrification -- Nitrogen removal performance -- Microbial diversity changes -- MENs analysis; Pollution -- Periodicals; Pollution -- Environmental aspects -- Periodicals; Environmental Pollution -- Periodicals; Pollution -- Périodiques; Pollution -- Aspect de l'environnement -- Périodiques; Pollution -- Effets physiologiques -- Périodiques; Pollution; Pollution -- Environmental aspects; Periodicals; Electronic journals; 363.73
• DOI: 10.1016/j.envpol.2020.114190
• Languages: English
• ISSNs: 0269-7491

Abstract

Abstract: Organ carbon are often used to enhance denitrification in wastewater treatment. However, their possible effects on microbial interactions are very limited. In this work, an anaerobic ammonium oxidation (anammox) coupled with sulfur autotrophic/mixotrophic denitrification (SAD/SMD) system was used to investigate the changes in microbial interactions among the microbial communities under different nutrient condition. The removal efficiency of total nitrogen increased from 70% (SAD) to 97% (SMD). The Illumina sequencing analysis indicated that Planctomycetes was the most dominant bacterial phylum in anammox system. Thiobacillus and Sulfurimonas, two typical autotrophic denitrifiers, decreased significantly from 31.9% to 17.7%–12.2% and 9.3%, when the nutrient condition changed from SAD to SMD ( P < 0.05). Meanwhile, some heterotrophic or mixotrophic denitrifying bacteria, including Gemmobacter, Pseudomonas and Thauera increased significantly ( P < 0.05). Molecular ecological network (MEN) analysis showed that the addition of organic carbon substantially altered the overall architecture of the network. Compared with SAD, the SMD had shorter path lengths, indicating higher transfer efficiencies of information and materials among different microorganism. The addition of organic carbon increased the microbial interaction complexity of Proteobacteria . The links of Thiobacillus, which was a typical sulfur-oxidizing autotrophic denitrifying bacteria, significantly reduced ( P < 0.05) with the addition of organic carbon, while the links of the heterotrophic bacteria Geobacter significantly increased ( P < 0.05). This study provided new insights into our understanding of the shifts in the bacteria community and their microbial interactions under different nutrient conditions (SAD and SMD) in sulfur-supported denitrification system. Graphical abstract: Image 1 Highlights: The removal efficiency of TN increased from 70% (SAD) to 97% (SMD). Organic carbon altered the diversity and the structure of bacterial community. Organic carbon increased the microbial interaction complexity of Proteobacteria . Thiobacillus links reduced while Geobacter links increased from SAD to SMD.