Molecular characterization of Latin American invasive Streptococcus pneumoniae serotype 19A isolates. Issue 19 (23rd April 2020)

• Record Type: Journal Article
• Authors: Almeida, Samanta Cristine Grassi; Vega, Mariana López; Chamorro, Gustavo A.; Kawabata, Anibal; Martín, Yolanda Narváez San; Morales, Sara; Revollo, Carmen; de Bolaños, Raquel; Gabastou, Jean Marc; Agudelo, Clara Inés; Castañeda, Elizabeth; Moreno, Jaime; Duarte, Carolina; Cassiolato, Ana Paula; Chacón, Grettel Chanto; Alarcon, Pedro; Sánchez, Jacqueline; Martín, Yolanda Narváez San; Valenzuela, Claudia; Castillo, Wendy; Gabarrot, Gabriela García
• Is Part Of: Vaccine. Volume 38:Issue 19(2020)
• Page Start: 3524
• Page End: 3530
• Publication Date: 2020-04-23
• Subjects: Streptococcus pneumoniae -- Invasive pneumococcal disease -- Latin America -- Serotype 19A -- Laboratory surveillance; Vaccines -- Periodicals; 615.372
• DOI: 10.1016/j.vaccine.2020.03.030
• Languages: English
• ISSNs: 0264-410X

Abstract

Highlights: The study describes genetic diversity of S. pneumoniae serotype 19A isolates in 13 Latin American countries. The most common sequence types (ST) of S. pneumoniae serotype 19A isolates were ST320, ST199, ST172 and ST5204. The study showed the expansion of international clones and the spread of minor clones in some countries. Abstract: Streptococcus pneumoniae is a major cause of morbidity and mortality worldwide, especially among children and the elderly. S. pneumoniae serotype 19A has emerged as a major cause of invasive disease in many countries, regardless of whether pneumococcal conjugate vaccines are used. The aim of this study was molecular characterization of invasive S. pneumoniae serotype 19A isolates recovered between 2000 and 2015 from 13 National Laboratories through the laboratory-based surveillance of invasive S. pneumoniae program SIREVA II in Latin American countries. The isolates were submitted with antimicrobial susceptibility tests and were genotyped by a combination of pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Of the 185 isolates assayed, notable rates of resistance to penicillin (MIC ≥ 0.125 µg/mL; 68.6%), tetracycline (63.7%), trimethoprim-sulfamethoxazole (63.2%), and erythromycin (43.2%) were observed, while 44.3% of isolates were multidrug resistant. The most frequently observed sequence types (ST) were ST320 (32.4%), ST199 (14.1%), ST172 (10.8%) and ST5204 (7.1%). The distribution of STs indicated regional differences in the epidemiology of the clonal groups. The present study showed a diverse genetic background of the pneumococcal population in Latin American countries. Continuous surveillance of the pneumococcal serotype 19A population in the region will be necessary to obtain information about geographical differences and changes in the spread and the establishment of particular clones.