Mutation of Arthrospira platensis by gamma irradiation to promote phenol tolerance and CO2 fixation for coal-chemical flue gas reduction. Issue 38 (May 2020)

• Record Type: Journal Article
• Authors: Zhu, Yanxia; Cheng, Jun; Zhang, Ze; Liu, Jianzhong
• Is Part Of: Journal of CO₂ utilization. Issue 38(2020)
• Page Start: 252
• Page End: 261
• Publication Date: 2020-05
• Subjects: Arthrospira platensis -- Phenol -- Flue gas -- Gamma ray -- Mutant; Carbon dioxide -- Periodicals; Carbon dioxide -- Environmental aspects -- Periodicals; Carbon dioxide mitigation -- Periodicals; Carbon dioxide; Carbon dioxide -- Environmental aspects; Carbon dioxide mitigation; Periodicals; 628.53205
• DOI: 10.1016/j.jcou.2020.02.003
• Languages: English
• ISSNs: 2212-9820

Abstract

Highlights: Phenol-tolerant and fast-growing A. platensis mutant was obtained with γ irradiation. The phenol degradation efficiency of mutant was 34.7 % higher than that of wild strain. β-ketoadipate pathway played a dominant role during phenol degradation in mutant. The CO2 fixation rate of mutant with phenol was 22.4 % higher than that of wild strain. Abstract: In order to promote phenol tolerance and the carbon fixation rate of cells during CO2 sequestration in coal chemical flue gas, γ-ray irradiation from cobalt-60 ( 60 Co) was used to mutate Arthrospira platensis to generate efficient strains. A phenol-tolerant strain Mutant 11k was obtained with 11, 000 Gy of gamma irradiation. The phenol degradation efficiency of the mutant was 34.7 % higher than that of wild strain, attributing to the increased activity of phenol hydroxylase that catalyzed phenol to generate catechol. The catechol 1, 2-dioxygenase activity in mutant cells was enhanced by 39.5 % while catechol 2, 3-dioxygenase was weakened by 23.8 %, revealing that the β-ketoadipate pathway plays a more important role than 2-hydroxymuconic semialdehyde pathway in phenol degradation. The activity of superoxide dismutase and catalase increased by 6.1 % and 39.8 %, respectively, reducing peroxide damage and maintaining rapid cell growth. The contents of chlorophyll-a and maximum quantum yield of photosystem II in mutant cells were higher than those in wild strain. The biomass yield and carbon fixation rate of mutant were 20.8 % and 22.4 % higher, respectively, than those of the wild strain.