Effect of glucoraphanin from broccoli seeds on lipid levels and gut microbiota in high-fat diet-fed mice. (May 2020)

• Record Type: Journal Article
• Authors: Xu, Xinxing; Dai, Mei; Lao, Fei; Chen, Fang; Hu, Xiaosong; Liu, Yuping; Wu, Jihong
• Is Part Of: Journal of functional foods. Volume 68(2020)
• Publication Date: 2020-05
• Subjects: Glucoraphanin -- Obesity -- Lipid metabolism -- Gut microbiota; HFD high-fat diet -- HPLC high-performance liquid chromatography -- ND normal diet -- HFDB broccoli-treated mice -- HFDG glucoraphanin-treated mice -- OGTT oral glucose tolerance test -- HOMA-IR homeostasis model assessment of insulin resistance -- TC total serum cholesterol -- TG total triglycerides -- HDL-C high-density lipoprotein cholesterol -- LDL-C low-density lipoprotein cholesterol -- AST aspartate transaminase -- ALT alanine transaminase -- MDA malondialdehyde -- SOD superoxide dismutase -- CAT catalase -- CPT-1 carnitine palmitoyl transterase-1 -- PPARα peroxisome proliferator-activated receptor alpha -- FAS fatty acid synthase -- SREBP-1c sterol regulatory element-binding protein-1c -- ACC acetyl-CoA carboxylase -- ACOX peroxisomal acyl-coenzyme A oxidase -- GAPDH glyceraldehyde-3-phosphate dehydrogenase -- OTU operational taxonomic unit -- LEfSe linear discriminant analysis effect size; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2020.103858
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: Glucoraphanin showed the highest bioaccessibility after in vitro gastrointestinal digestion. Glucoraphanin modulated lipid profiles and metabolism related gene expression. Glucoraphanin supplementation changed the composition and richness of gut microbiota. Correlation analysis between biochemical factors and gut microbes was investigated. Abstract: Glucoraphanin was found to be the most abundant glucosinolate both in crude broccoli and after in vitro gastrointestinal digestion, with a bioaccessibility of 46.2 ± 0.4%. Glucoraphanin has been reported as a stable precursor of the nuclear factor (erythroid-derived 2)-like 2 (Nrf2) inducer sulforaphane which correlated with obesity. Thus, the effects of glucoraphanin on lipid metabolism and gut microflora in high-fat diet-fed mice were investigated. Glucoraphanin decreased liver weights and adipose tissue masses, concentrations of serum inflammatory factors, and the expression of genes of FAS and increased the expression of genes of PPARα, CPT1, ACOX in the liver. Glucoraphanin also decreased the Firmicutes / Bacteroidetes ratio and the abundance of microbes positively correlated with the high-fat diet in intestinal microbiota. The findings illustrate that glucoraphanin is a potential anti-obesity potential substance and suggest the utilization of seed flours and bio-products of glucoraphanin-rich cruciferous vegetables in nutraceuticals and functional foods.