Anaerobic digestion of aqueous phase from hydrothermal liquefaction of Spirulina using biostimulated sludge. (September 2020)

• Record Type: Journal Article
• Authors: Egerland Bueno, Beatriz; Américo Soares, Laís; Quispe-Arpasi, Diana; Kimiko Sakamoto, Isabel; Zhang, Yuanhui; Amancio Varesche, Maria Bernadete; Ribeiro, Rogers; Tommaso, Giovana
• Is Part Of: Bioresource technology. Volume 312(2020)
• Publication Date: 2020-09
• Subjects: Toxic wastewaters -- Biodegradation -- PCR/DGGE -- 16S rDNA sequencing -- Taxonomical potential; AD anaerobic digestion -- B biostimulated sludge -- BCO biocrude oil -- COD chemical oxygen demand -- GC gas chromatography -- HTL hydrothermal liquefaction -- NB non-biostimulated sludge -- PHWW post-hydrothermal liquefaction wastewater, total solids -- TVS total volatile solids -- VSS volatile suspended solids; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2020.123552
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: Mesotoga and Methanomethylovorans were favoured by biostimulation process. Biostimulation process favoured the degradability of PHWW. Higher methane production was obtained at of 10 and 16 g COD.L −1 of PHWW. Higher maximum specific methane production rates were observed after biostimulation. Abstract: Hydrothermal liquefaction is a process that converts wet biomass into biofuels, more specifically bio-crude oil. During the process, post hydrothermal liquefaction waste water (PHWW) is generated, rich in nutrient and organic matter, however potentially toxic. Anaerobic digestion of PHWW from Spirulina, was evaluated using biostimulated sludge as a strategy to optimize the process. The biostimulation was conducted in a sequential batch reactor fed with organic acids and methanol aiming at development of acetogenic and methanogenic microorganism. Anaerobic biodegradability batch assays were performed, with biostimulated sludge and with non-biostimulated sludge, using increasing PHWW concentrations. Biostimulated sludge were more favourable for reaching higher methane yields at higher organic matter concentrations in comparison to non-biostimulated sludge, presenting less inhibition at conditions tested. Biostimulation was a key process to select and favour potential microorganisms involved in specialized uptake of recalcitrant compounds, such as Mesotoga and Methanomethylovorans .