Expression and functional analysis of Nile tilapia transferrin receptors (TfRs) in host resistance to pathogenic bacteria and iron ion metabolism. Issue 100 (May 2020)

• Record Type: Journal Article
• Authors: Yin, Xiaoxue; Mu, Liangliang; Wu, Hairong; Han, Kailiang; Guo, Zheng; Ye, Jianmin
• Is Part Of: Fish & shellfish immunology. Issue 100(2020)
• Page Start: 407
• Page End: 417
• Publication Date: 2020-05
• Subjects: Oreochromis niloticus -- Transferrin receptor -- Host resistance -- Pathogenic bacteria -- Iron ion; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2020.03.027
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Transferrin receptors (TfRs) play an essential role in iron-withholding strategy, and are involved in immune response against bacterial infection. In this study, the transferrin receptor 1 ( OnTfR1 ) and transferrin receptor 2 ( OnTfR2 ) genes are identified and characterized in Nile tilapia ( Oreochromis niloticus ). The open reading frames of OnTfR1 and OnTfR2 are 2220 and 2343 bp of nucleotide sequence, encoding 739 and 780 amino acids, respectively. The deduced proteins of OnTfR1 and OnTfR2 are highly homologous to those of other species, containing three conserved TfR superfamily domains (PA TfR domain, M28 TfR domain and TfR dimer domain). Expression analyses of OnTfRs in the healthy tilapia reveal that the OnTfR1 and OnTfR2 transcripts are the most abundant in the liver. The in vivo studies show that the expressions of OnTfRs are significantly up-regulate in liver and spleen, following infections of Streptococcus agalactiae and Aeromonas hydrophila . In addition, the in vitro studies reveal that the up-regulations of OnTfR expressions are also significant in monocytes/macrophages and hepatocytes upon the stimulations of S. agalactiae and A. hydrophila . Moreover, the iron ion (Fe 3+ ) could significantly increase the expressions of OnTfRs in monocytes/macrophages and hepatocytes. Taken together, the present study indicates that OnTfRs may be involved in host defense against bacterial infection and possess the function of combining or transporting iron ions in Nile tilapia. Highlights: The transcripts of OnTfRs were significantly up-regulated upon the challenge of pathogenic bacteria in vivo and in vitro. The OnTfRs could quickly respond to iron stress and the expression was regulated by the concentration of iron ion. The OnTfR1 mRNA was more sensitive to iron ion stimulation.