Three-week joint immobilization increases anterior-posterior laxity without alterations in mechanical properties of the anterior cruciate ligament in the rat knee. (May 2020)

• Record Type: Journal Article
• Authors: Kaneguchi, Akinori; Ozawa, Junya; Minamimoto, Kengo; Yamaoka, Kaoru
• Is Part Of: Clinical biomechanics. Volume 75(2020)
• Publication Date: 2020-05
• Subjects: Joint immobilization -- Joint laxity -- Anterior cruciate ligament -- Biomechanical properties -- Joint capsule; Biomechanics -- Periodicals; Osteopathic medicine -- Periodicals; Biomechanics -- Periodicals; Osteopathic Medicine -- Periodicals; 612.76
• DOI: 10.1016/j.clinbiomech.2020.104993
• Languages: English
• ISSNs: 0268-0033

Abstract

Abstract: Background: Although knee immobilization may deteriorate the mechanical parameters of the anterior cruciate ligament, such as stiffness and failure strength, it is unknown whether it induces laxity in the whole joint. We examined the effects of immobilization on anterior-posterior joint laxity and mechanical properties of the anterior cruciate ligament, as well as histological and gene expression profiles of the joint capsule in rat knee joints. Methods: Unilateral rat knees were immobilized using an external fixator. Non-immobilized contralateral knees were used as controls. After 3 weeks, anterior-posterior laxity in the whole joint (i.e., a complex of bones, ligaments, and capsule) and stiffness and failure strength in the anterior cruciate ligament were examined using a universal testing machine. Moreover, the knee joint capsule was histologically analyzed, and the expression levels of genes related to collagen turnover in the posterior joint capsule were examined. Findings: Joint immobilization slightly but significantly increased anterior-posterior laxity compared with the contralateral side. Unexpectedly, the stiffness and failure strength of the anterior cruciate ligament were not altered by immobilization. There was no correlation found between anterior cruciate ligament stiffness and anterior-posterior joint laxity. In the posterior joint capsule, thinning of the collagen fiber bundles accompanied by a decrease in COL3A1 gene expression was observed after immobilization. Interpretation: These results suggest that 3 weeks of joint immobilization alters the biomechanical integrity in the knee joint without altering the mechanical properties of the anterior cruciate ligament. Changes in the joint capsule may contribute to the immobilization-induced increase in anterior-posterior laxity. Highlights: Joint immobilization may deteriorate the mechanical parameters of the ligament. However, it is unknown whether immobilization induces laxity in the whole joint. Immobilization slightly but significantly increased anterior-posterior laxity. The mechanical parameters of the anterior cruciate ligament were not altered. Joint capsule may contribute to the immobilization-induced joint laxity.