Development of regression model for bacteriocin production from local isolate of Lactobacillus acidophilus MS1 using Box-Behnken design. (March 2020)

• Record Type: Journal Article
• Authors: Salman, Mahwish; Shahid, Muhammad; Sahar, Tanzila; Naheed, Shazia; Mahmood-ur-Rahman, ; Arif, Muhammad; Iqbal, Munawar; Nazir, Arif
• Is Part Of: Biocatalysis and agricultural biotechnology. Number 24(2020)
• Publication Date: 2020-03
• Subjects: Lactobacillus acidophilus -- Bacteriocin -- Response surface methodology -- Box-Behnken design; Agricultural biotechnology -- Periodicals; Enzymes -- Biotechnology -- Periodicals; 660.6
• DOI: 10.1016/j.bcab.2020.101542
• Languages: English
• ISSNs: 1878-8181

Abstract

Abstract: Bacteriocins are antimicrobial peptides synthesized by lactic acid bacteria. These are well known for their anti-bacterial activity in fermented foods and extend the shelf-life and safety of food items. The optimum bacteriocin production was predicted from Lactobacillus acidophilus MS1 using Box-Behnken experimental design. The maximum bacteriocin activity titre of 2600AU/mL was observed with 30 °C, pH 6 and 18 h incubation time. The results derived from response surface methodology (RSM) regression equation indicated that the initial pH of the model system influencing stronger than other physical variables at 95% confidence level. Moreover, quadratic and interaction effects of these variables were also found significant. A monomeric peptide of 6.5 kDa was purified to 59.1-fold from crude sample using ion exchange and gel filtration chromatography. Purified peptide showed strong inhibitory activity against biofilm produced by Bacillus cereus, a food born pathogen. Box-Behnken design can be used for large scale production of bacteriocin from novel strain of Lactobacillus acidophilus MS1 that might be used as a preservative and non-allergic antibiotic in food as well as in pharmaceuticals. Highlights: Bacteriocins are synthesized by lactic acid bacteria for safety of food items. Bacteriocin production was projected from L. acidophilus MS1 using Box-Behnken design. Maximum bacteriocin activity was observed with 30 °C temperature, pH 6 and 18 h incubation. RSM exhibited initial pH of the model system has strong influence at 95% confidence level. Monomeric peptide of 6.5 kDa was purified to 59.1-fold using chromatographic methods.