Optimal timing of apheresis for the efficient mobilization of peripheral blood progenitor cells recruited by high-dose granulocyte colony-stimulating factor in healthy donors. (June 2020)

• Record Type: Journal Article
• Authors: Kimura, Satoshi; Ohkawara, Hiroshi; Minakawa, Keiji; Fukatsu, Masahiko; Mori, Hirotaka; Takahashi, Hiroshi; Harada-Shirado, Kayo; Ohara, Yoshihiro; Takahashi, Nobuhisa; Mochizuki, Kazuhiro; Sano, Hideki; Nollet, Kenenth E; Ogawa, Kazuei; Ohto, Hitoshi; Kikuta, Atsushi; Ikeda, Kazuhiko; Ikezoe, Takayuki
• Is Part Of: Transfusion and apheresis science. Volume 59:Number 3(2020)
• Publication Date: 2020-06
• Subjects: Apheresis -- Peripheral blood stem cells -- Poor mobilization -- Cytomegalovirus; Blood -- Transfusion -- Periodicals; Hemapheresis -- Periodicals; 615.39
• DOI: 10.1016/j.transci.2020.102737
• Languages: English
• ISSNs: 1473-0502

Abstract

Abstract: Predictors of peripheral blood stem cell (PBSC) yield can potentially improve the comfort, safety, and efficacy of CD34+ cell collection from donors treated with recombinant human granulocyte colony-stimulating factor (G-CSF). We investigated 181 apheresis procedures on 109 healthy allogeneic donors to identify factors correlating with efficient PBSC collection. Apheresis started on Day 4 or 5 and continued up to Day 6 of G-CSF administration. CD34+ cell yields on Days 4 and 5 were comparable, and significantly higher than on Day 6. This suggests that starting apheresis on Day 4 rather than Day 5 may be preferable, to reduce G-CSF exposure and optimize yield, even if multi-day collection is required. More CD34+ cells were collected from male and cytomegalovirus (CMV)-seronegative donors than from female and CMV-seropositive donors, respectively. The yields of CD34+ cells were similarly high in both male and female donors aged 20–29 years; yields decreased in female donors in their thirties, and were comparably low in both male and female donors in their forties and thereafter. These findings should guide decision-making about when to begin apheresis, and encourage careful consideration of donor factors such as gender, age, and CMV serostatus when collecting PBSCs.