Enhanced selective enrichment of partial nitritation and anammox bacteria in a novel two-stage continuous flow system using flat-type poly (vinylalcohol) cryogel films. (March 2020)

• Record Type: Journal Article
• Authors: Choi, Minkyu; Chaudhary, Ramjee; Lee, Minjoo; Kim, Jihyun; Cho, Kyungjin; Chung, Yun-Chul; Bae, Hyokwan; Park, Joonhong
• Is Part Of: Bioresource technology. Volume 300(2020)
• Publication Date: 2020-03
• Subjects: Anammox Anaerobic ammonia oxidation -- AOB Ammonia oxidizing bacteria -- AOR Ammonia oxidation rate -- AS Activated sludge -- BOD Biological oxygen demand -- BNR Biological nitrogen removal -- COD Chemical oxygen demand -- DO Dissolved oxygen -- FA Free ammonia -- FBBR Fixed-bed bioreactor -- FT-CPVAF Flat-type PVA cryogel film -- HRT Hydraulic retention time -- NCBI National Centre for Biotechnology Information -- NLR Nitrogen loading rate -- NOB Nitrite oxidizing bacteria -- NRE Nitrogen removal efficiency -- NRR Nitrogen removal rate -- OTU Operational taxonomic unit -- PAB Pre-enriched anammox bacteria -- PCR Polymerase chain reaction -- PN Partial nitritation -- PVA Poly (vinylalcohol) -- SBR Semi-batch reactor -- VSS Volatile suspended solid -- WWTP Wastewater treatment plant; Partial nitritation -- Anammox -- Immobilization -- PVA cryogel -- Reactor microbiome; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2019.122546
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Stable PN with high AOR 1.68 ± 0.12 kg N m −3 d −1 in short acclimation of 8 days. Flat-type PVAcryogel elevated endurance of immobilized anammox bacteria to O2 shock. A novel two-stage PN-anammox system achieved 90% N removal at short HRT of 7.7 h. Microbial analysis confirmed enhanced selective growth of AOB and anammox bacteria. Abstract: To improve stability of nitrogen removal in partial nitritation (PN)-anammox process, flat-type cryogel films using poly (vinylalcohol) named as FT-CPVAF were applied in continuous reactors. Stable PN operation was maintained with short acclimation of 8 days and ammonium oxidation rate of 1.68 ± 0.12 kg N m −3 d −1 comparatively higher than previous studies. The nitrogen removal, initially inhibited by an oxygen shock, was immediately reactivated with short lag-period by immobilization of anammox bacteria in FT-CPVAF. A novel two-stage PN-anammox process was operated in a continuous flow using FT-CPVAF for treatment of ammonium-rich synthetic wastewater (influent 315 mg NH4 + -N L −1 ) showing 89.6 ± 0.76% of nitrogen removal at short hydraulic retention time (7.7 h). The use of FT-CPVAF enhanced selective enrichment of AOB and anammox bacter ia confirmed by high-throughput sequencing of i.e., relative abundances of Nitrosomonas europaea C-31 (37.14% in PN reactor) and ' Candidatus Jettenia caeni' (34.36% in anammox reactor).