Inhibition of biofilm and virulence properties of Pseudomonas aeruginosa by sub-inhibitory concentrations of aminoglycosides. (September 2020)

• Record Type: Journal Article
• Authors: Khan, Fazlurrahman; Lee, Jang-Won; Javaid, Aqib; Park, Seul-Ki; Kim, Young-Mog
• Is Part Of: Microbial pathogenesis. Volume 146(2020)
• Publication Date: 2020-09
• Subjects: Aminoglycosides -- Antibiofilm -- Anti-virulence -- LasR -- Molecular docking -- Pseudomonas aeruginosa; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2020.104249
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: Aminoglycosides are a commonly used class of antibiotics; however, their application has been discontinued due to the emergence of multi-drug resistance bacterial strains. In the present study, the subinhibitory concentrations (sub-MIC) of several aminoglycosides were determined and tested as an antibiofilm and for their anti-virulence properties against Pseudomonas aeruginosa PAO1, which is an opportunistic foodborne pathogen. P. aeruginosa PAO1 exhibits multiple mechanisms of resistance, including the formation of biofilm and production of several virulence factors, against aminoglycoside antibiotics. The sub-MIC of these antibiotics exhibited biofilm inhibition of P. aeruginosa in alkaline TSB (pH 7.9). Moreover, various concentrations of these aminoglycosides also eradicate the mature biofilm of P. aeruginosa . In the presence of sub-MIC of aminoglycosides, the morphological changes of P. aeruginosa were found to change from rod-shaped to the filamentous, elongated, and streptococcal forms. Similar growth conditions and sub-MIC of aminoglycosides were also found to attenuate several virulence properties of P. aeruginosa PAO1. Molecular docking studies demonstrate that these aminoglycosides possess strong binding properties with the LasR protein, which is a well-characterized quorum-sensing receptor of P. aeruginosa . The present study suggests a new approach to revitalize aminoglycosides as antibiofilm and antivirulence drugs to treat infections caused by pathogenic bacteria. Highlights: P. aeruginosa PAO1 exhibits multiple resistance mechanisms against aminoglycosides. Sub-MIC of aminoglycosides exhibited inhibition and eradication of biofilm. Sub-MIC of aminoglycosides attenuates QS-mediated virulence properties. Aminoglycosides changes the morphology of the P. aeruginosa.