A novel multilocus sequence typing scheme identifying genetic diversity amongst Leishmania donovani isolates from a genetically homogeneous population in the Indian subcontinent. Issue 7 (June 2019)

• Record Type: Journal Article
• Authors: Banu, Sultana Shahana; Meyer, Wieland; Ferreira-Paim, Kennio; Wang, Qinning; Kuhls, Katrin; Cupolillo, Elisa; Schönian, Gabriele; Lee, Rogan
• Is Part Of: International journal for parasitology. Volume 49:Issue 7(2019)
• Page Start: 555
• Page End: 567
• Publication Date: 2019-06
• Subjects: Leishmania donovani -- Genetic diversity -- Multilocus sequence typing -- Indian subcontinent -- Bangladesh; Parasitology -- Periodicals; Parasitology -- Periodicals; Parasitologie -- Périodiques; Parasitology; Periodicals; Electronic journals; 571.999
• DOI: 10.1016/j.ijpara.2019.02.010
• Languages: English
• ISSNs: 0020-7519

Abstract

Graphical abstract: Highlights: Seven new multi-locus sequence typing (MLST) loci separate Leishmania donovani from Leishmania infantum . A2P is the most discriminative locus used in the proposed multilocus sequence typing scheme. Seven genotypes were identified amongst very homogeneous Bangladeshi isolates. Bangladeshi isolates differ from isolates from India, Nepal, Iraq and Africa. A new MLST approach detects intra- and inter-species variations in the L. donovani complex. Abstract: In the Indian subcontinent, infection with Leishmania donovani can cause fatal visceral leishmaniasis. Genetic variation in L. donovani is believed to occur rapidly from environmental changes and through selective drug pressures, thereby allowing continued disease occurrence in this region. All previous molecular markers that are commonly in use multilocus microsatellite typing and multilocus sequence typing, were monomorphic in L. donovani originating from the Indian subcontinent (with only a few exceptions) and hence are not suitable for this region. An multilocus sequence typing scheme consisting of a new set of seven housekeeping genes was developed in this study, based on recent findings from whole genome sequencing data. This new scheme was used to assess the genetic diversity amongst 22 autochthonous L. donovani isolates from Bangladesh. Nineteen additional isolates of the L. donovani complex (including sequences of L. donovani reference strain BPK282A1) from other countries were included for comparison. By using restriction fragment length polymorphism of the internal transcribed spacer 1 region (ITS1-RFLP) and ITS1 sequencing, all Bangladeshi isolates were confirmed to be L. donovani . Population genetic analyses of 41 isolates using the seven new MLST loci clearly separated L. donovani from Leishmania infantum . With this multilocus sequence typing scheme, seven genotypes were identified amongst Bangladeshi L. donovani isolates, and these isolates were found to be phylogenetically different compared with those from India, Nepal, Iraq and Africa. This novel multilocus sequence typing approach can detect intra- and inter-species variations within the L. donovani complex, but most importantly these molecular markers can be applied to resolve the phylogenetically very homogeneous L. donovani strains from the Indian subcontinent. Four of these markers were found suitable to differentiate strains originating from Bangladesh, with marker A2P being the most discriminative one.