A novel lignin degradation bacteria-Bacillus amyloliquefaciens SL-7 used to degrade straw lignin efficiently. (August 2020)

• Record Type: Journal Article
• Authors: Mei, Jinfei; Shen, Xiaobao; Gang, Liping; Xu, Huajie; Wu, Fufang; Sheng, Liangquan
• Is Part Of: Bioresource technology. Volume 310(2020)
• Publication Date: 2020-08
• Subjects: B. amyloliquefaciens Bacillus amyloliquefaciens -- COD Chemical Oxygen Demand -- Lip Lignin peroxidase -- MnP Manganese peroxidase -- Lac Laccase -- Ts Tobacco straw -- LB Luria-Bertani -- OD600 Optical density at 600 nm -- SEM Scanning electron microscope -- ABTS 2, 2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) -- 2, 6-DMP 2, 6-dimethyl-phenol -- OD Optical density -- KB Uninoculated agent -- GI Germination index -- HS Humus -- WG Effective Microorganisms -- GDMCC Guangdong Microbial Culture Center; Tabacco straw -- Bacillus amyloliquefaciens SL-7 -- Biodegradation -- Compost; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2020.123445
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: The first report on tobacco straw lignin degradation by bacterium of genus Bacillus . Bacillus amyloliquefaciens SL-7 secreted Lip, MnP and Lac to degrade lignin. The practical application effect of this strain on tobacco straw compost was explored. Abstract: Using tobacco straw (Ts) and lignin as the sole carbon source, a strain was isolated from Ts and identified as Bacillus amyloliquefaciens SL- 7 by 16S rDNA gene-sequencing technology.7-day incubation of Bacillus amyloliquefaciens SL- 7 can reduce the chemical oxygen demand (COD) by 69.35% in lignin mineral salt medium. The activity of Manganese peroxidase (MnP) reached maximum level 258.57 U L −1, and Lignin peroxidase (Lip) was 422.68 U L −1 at 4th day. The highest Laccase (Lac) activity (55.95 U L −1 ) was observed at 3th day. After straw-liquid fermentation degradation of 15 days, the bacterial could degrade 28.55% lignin of the straw which was close to that of fungi. Compared with the control group and effective microorganisms (EM) group, the lignin degradation rate in Bacillus amyloliquefaciens SL-7 group increased by 22.26% and 11.70% at 41-day compost fermentation of tobacco straw. These show the strain has strong lignin degradation performance.