LncRNA TUG1 regulates ulcerative colitis through miR-142-5p/SOCS1 axis. (June 2020)

• Record Type: Journal Article
• Authors: Han, Jing; Li, Yawei; Zhang, Baolian; Liu, Hong; Wu, Mengyao; Zhang, Xiaolan
• Is Part Of: Microbial pathogenesis. Volume 143(2020)
• Publication Date: 2020-06
• Subjects: Long non-coding RNA -- TUG1 -- Ulcerative colitis -- miR-142-5p -- SOCS1; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2020.104139
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: Ulcerative colitis (UC) is a long-lasting inflammation disease which finally results in ulcer of the colon and rectum. The long non-coding RNA (lncRNA) TUG1 has been described to target miR-142 and regulate its expression. In current study, we evaluated the effects of long non-coding RNA TUG1 on cell injury and inflammatory cytokine production using a TNFα-treated HT-29 cells model. We monitored the level of TUG1 in colonic mucosa tissue of UC patients and in TNF-α-treated HT-29 cells. We investigated the effects of TUG1 on miR-142-5p and SOCS1expression, cell viability, lactate dehydrogenase (LDH) release, production of nitrite and PGE2 after TNF-α treatment in HT-29 cells. We also investigated the effects of TUG1 on TNF-α-induced IL-6, IL-8 and IL-1β expression in HT-29 cells. We detected down-regulated TUG1 level in colonic mucosa tissue of UC patients and in TNF-α-treated HT-29 cells. Overexpression of TUG1 enhanced cell viability, decreased LDH release, decreased nitrite and PGE2 production after TNF-α treatment in HT-29 cells. TUG1 prevented IL-1β, IL-6 and IL-8 production in TNF-α-treated cells. TUG1 targeted miR-142-5p and inhibited its expression while enhanced SOCS1 expression. Overexpression of miR-142-5p abolished TUG1-mediated inhibition of TNF-induced inflammatory cytokines production. TUG1 negatively regulated inflammation in ulcerative colitis through miR-142-5p/SOCS1 axis. Highlights: TUG1 level was down-regulated in UC patients. TUG1 overexpression inhibited inflammatory events in UC. TUG1 negatively regulated inflammation in UC through miR-142-5p/SOCS1 axis.