Self-assembled hyaluronic acid nanoparticle suppresses fat accumulation via CD44 in diet-induced obese mice. (1st June 2020)

• Record Type: Journal Article
• Authors: Lee, Wang Hee; Rho, Jun Gi; Han, Hwa Seung; Kweon, Sohui; Nguyen, Van Quy; Park, Jae Hyung; Kim, Wook
• Is Part Of: Carbohydrate polymers. Volume 237(2020)
• Publication Date: 2020-06-01
• Subjects: Hyaluronic acid -- Self-assembled nanoparticles -- Adipogenesis -- Lipogenesis -- Obesity; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2020.116161
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: HA-NPs suppressed adipogenesis and lipid accumulation. HA-NPs decreased the expression of key adipogenic and lipogenic regulators. The epididymal fat mass of DIO mice was reduced by HA-NP. These biological effects of HA-NPs were mediated by CD44. Abstract: Obesity, a major risk factor for type 2 diabetes and cardiovascular diseases, is characterized by an abnormal expansion of adipose tissue. Herein, we investigated the potential of hyaluronic acid nanoparticles (HA-NPs) as therapeutics to treat obesity-related diseases by assessing the in vitro and in vivo effects of HA-NPs on adipogenesis and lipogenesis. Treatment of 3T3-L1 preadipocytes with HA-NPs resulted in a dose-dependent suppression of adipogenesis and lipid accumulation, and decreased the expression of key adipogenic and lipogenic regulators. However, these HA-NPs mediated effects were not observed in 3T3-L1 cells transfected with siRNAs against CD44, a major HA receptor. Further, HA-NP treatment of diet-induced obese (DIO) mice reduced the epididymal fat mass and suppressed the induction of adipogenic and lipogenic regulators, while these effects were attenuated in the CD44 -null mice. Thus, our study provides a better understanding of how HA-NP modulates fat accumulation and presents a potential anti-obesity strategy targeting CD44.