Determination of BCM-7 based on an ultrasensitive aptasensor fabricated of gold nanoparticles and ZnS quantum dots. (June 2020)

• Record Type: Journal Article
• Authors: Roushani, Mahmoud; Farokhi, Somayeh; Shahdost-fard, Faezeh
• Is Part Of: Materials today communications. Volume 23(2020)
• Publication Date: 2020-06
• Subjects: β-casomorphin 7 -- Autism -- Gold nanoparticles -- Aptasensor -- Thiourea capped-ZnS QDs -- Glassy carbon electrode; Materials science -- Periodicals; 620.11
• DOI: 10.1016/j.mtcomm.2020.101066
• Languages: English
• ISSNs: 2352-4928

Abstract

Graphical abstract: Highlights: An AuNPs@ZnS-QDs nanocomposite as an efficient platform was presented in BCM-7 detection. The BCM-7 as a target was strongly attached to the Apt as a capture onto the aptasensor surface. The proposed strategy had the lowest detection limit for the BCM-7 determination in comparison to other related methods. The aptasensor for BCM-7 detection in human urine was successfully evaluated. The results offer this strategy may aid future efforts in the diagnosis of autism symptoms. Abstract: In this study, an ultrasensitive electrochemical aptasensor for the determination of the β-casomorphin 7 (BCM-7) as a promising biomarker of autism disorder is introduced. According to the proposed strategy, a glassy carbon electrode (GCE) was modified by thiourea capped-ZnS QDs (ZnS-QDs) and gold nanoparticles (AuNPs) to further immobilization the amino-aptamer (NH2 -Apt) on its surface. The NH2 -Apt as a receptor of the BCM-7 was attached to the embedded surface via a formation of the Au–N bonds between the AuNPs and amino groups of the Apt. The evaluation of aptasensor by various electrochemical techniques exhibited successful sensing of the BCM-7 under two broad linear concentration ranges from 1 fM to 0.6 μM with a limit of detection (LOD) down to 350 aM. Also, the performance of the aptasensor in BCM-7 detection in real human urine samples was satisfactorily investigated. These findings may facilitate distinguish of this challenging disorder and help human health.