Profiling inflammatory response in lesions of cutaneous leishmaniasis patients using a non-invasive sampling method combined with a high-throughput protein detection assay. (June 2020)

• Record Type: Journal Article
• Authors: Taslimi, Yasaman; Agbajogu, Christopher; Brynjolfsson, Siggeir Fannar; Masoudzadeh, Nasrin; Mashayekhi, Vahid; Gharibzadeh, Safoora; Östensson, Malin; Nakka, Sravya Sowdamini; Mizbani, Amir; Rafati, Sima; Harandi, Ali M.
• Is Part Of: Cytokine. Volume 130(2020)
• Publication Date: 2020-06
• Subjects: Cutaneous leishmaniasis -- Non-invasive sampling -- Proximity extension assay -- Inflammation biomarkers; ADA Adenosine deaminase -- ARTN Artemin -- Beta-NGF Nerve growth factor -- CSF-1 Colony stimulating factor 1 -- CST5 Cystatin D -- DNER Delta/Notch Like EGF Repeat Containing -- 4E-BP1 Eukaryotic initiation factor 4E-binding protein 1 -- EN-RAGE LAP (TGF-beta-1) Advanced glycation end-products binding protein -- FGF Fibroblast growth factor -- GDNF Glial cell line-derived neurotrophic factor -- HGF Hepatocyte growth factor -- LIF Leukemia inhibitory factor, IL-6 family -- MCP-1 Monocyte chemoattractant protein-1 -- MMP Matrix metalloproteinase -- NRTN Neurturin -- OPG Osteoprotegerin, member of TNF family -- OSM Oncostatin M, IL-6 family -- PDL-1 Program death legend 1 -- ST1A1 Sulfotransferase1A1 -- STAMPB STAM binding protein -- SIR2 SIR 2 like protein 2 -- TNFRSF Tumor necrosis factor receptor superfamily member -- TGF-β Transforming growth factor-beta -- TRAIL TNF-related apoptosis-inducing ligand -- TRANCE TNF-related activation-induced cytokine -- uPA Urokinase-type plasminogen activator -- VEGFA Vascular Endothelial Growth Factor A; Cytokines -- Periodicals; 571.844
• DOI: 10.1016/j.cyto.2020.155056
• Languages: English
• ISSNs: 1043-4666

Abstract

Highlights: A non-invasive sampling method combined with a multiplexed protein assay was developed for evaluation of cytokine and chemokines in the lesions of cutaneous leishmaniasis patients. Various inflammatory cytokines, chemokines and other proteins were detected in the lesions of cutaneous leishmaniasis patients. This non-invasive sampling method combined with a multiplexed protein assay has implications for evaluation of skin inflammatory responses in cutaneous leishmaniasis. Abstract: Background: Cutaneous leishmaniasis (CL) is an infection caused by Leishmania ( L. ) protozoa transmitted through the bite of infected sand fly. Previously, invasive sampling of blood and skin along with low throughput methods were used for determination of inflammatory response in CL patients. Aims/Methodology: We established a novel approach based on a non-invasive adhesive tape-disc sampling combined with a powerful multiplexing technique called proximity extension assay for profiling 92 inflammatory cytokines, chemokines and surface molecules in the lesions of CL patients infected with L. tropica . Sample collection was done non-invasively by using adhesive tape-discs from lesion and normal skin of 33 L. tropica positive patients. Results: Out of 92 inflammatory proteins, the level of 34 proteins was significantly increased in the lesions of CL patients compared to their normal skin. This includes the chemokines CCL2, CCL3, CCL4, CXCL1, CXCL5, CXCL9, CXCL10 and CXCL11, together with the interleukins IL-6, IL-8, IL-18, LIF and OSM. The remaining significantly changed inflammatory proteins include 7 surface molecules and receptors: CD5, CD40, CDCP1, 4E-BP1, TNFRSF9, IL-18R1 and OPG as well as 16 other cytokines and proteins: MMP-1, CSF-1, VEGFA, uPA, EN-RAGE, LAP TGF-β1, HGF, MMP-10, CASP-8, TNFSF14, STAMPB, ADA, TRAIL and ST1A1. Further, 13 proteins showed an increasing trend, albeit not statistically significant, in the CL lesions, including TGF-α, CCL23, MCP-2, IL-12B, CXCL6, IL-24, FGF-19, TNFβ, CD6, TRANCE, IL10, SIR2 and CCL20. Conclusion: We herein report a novel approach based on a non-invasive sampling method combined with the high-throughput protein assay for profiling inflammatory proteins in CL lesions. Using this approach, we could profile inflammatory proteins in the lesions from CL patients. This new non-invasive approach may have implications for studying skin inflammatory mediators in CL and other skin disorders.