Elevated conversion of CO2 to versatile formate by a newly discovered formate dehydrogenase from Rhodobacter aestuarii. (June 2020)

• Record Type: Journal Article
• Authors: Min, Kyoungseon; Park, Yong-Soo; Park, Gwon Woo; Lee, Joon-pyo; Moon, Myounghoon; Ko, Chang Hyun; Lee, Jin-Suk
• Is Part Of: Bioresource technology. Volume 305(2020)
• Publication Date: 2020-06
• Subjects: CO2 reductase -- Rhodobacter aestuarii -- CO2 reduction -- Formate; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2020.123155
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: RaFDH is newly discovered through genome-mining and phylogenetic analysis. The recombinant RaFDH was characterized as a CO2 reductase under aerobic condition. In particular, RaFDH-driven CO2 reduction far surpassed formate oxidation. Abstract: Due to climate change, recent research interests have increased towards CO2 utilization as a strategy to mitigate the atmospheric CO2 level. Herein, we aimed to explore formate dehydrogenases (FDHs) from chemoautotroph to discover an efficient and O2 -tolerant biocatalyst for catalyzing the CO2 reduction to a versatile formate. Through genome-mining and phylogenetic analysis, the FDH from Rhodobacter aestuarii (RaFDH) was newly discovered as a promising O2 -tolernat CO2 reductase and was successfully expressed in Escherichia coli . In this study, the optimum conditions and turnover rates of RaFDH were examined for CO2 reduction and formate oxidation. In particular, the RaFDH-driven CO2 reduction far surpassed the formate oxidation with a turnover rate of 48.3 and 15.6 min −1, respectively. The outstanding superiority of RaFDH towards CO2 reduction can be applicable for constructing a feasible electroenzymatic system that produce a versatile formate from CO2 as a cheap, abundant, and renewable resource.