AOC4P suppresses viability and invasion and induces apoptosis in NSCLC cells by inhibiting the Wnt/β-catenin pathway. (1st July 2020)

• Record Type: Journal Article
• Authors: Li, Fengbo; Rong, Tao; Cao, Gang; Zhai, Chaoshuan; Li, Qian; Gong, Rui; Li, Gang
• Is Part Of: Chemico-biological interactions. Volume 325(2020)
• Publication Date: 2020-07-01
• Subjects: AOC4P -- Viability -- Invasion -- Apoptosis -- The Wnt/β-catenin pathway -- Non-small cell lung cancer; Biochemistry -- Periodicals; Toxicological chemistry -- Periodicals; Biochemistry -- Periodicals; Biologie moléculaire -- Périodiques; Biochimie -- Périodiques; Toxicologie biochimique -- Périodiques; 572
• DOI: 10.1016/j.cbi.2020.109110
• Languages: English
• ISSNs: 0009-2797

Abstract

Abstract: Increasing studies have well-documented the involvement of numerous lncRNAs in regulating the malignant phenotypes of various tumors including non-small cell lung cancer (NSCLC) cells. However, up to date, the effects and mechanism of lncRNA amine oxidase, copper containing 4, pseudogene (AOC4P) in NSCLC progression remain undefined. AOC4P expression in NSCLC cells was detected by qRT-PCR. The protein levels of Wnt/β-catenin pathway-related proteins, matrix metallopeptidase (MMP)-2, and MMP-9 were examined by Western blot. The effects of AOC4P or combined with Wnt agonist BML-284 on the malignant phenotypes in NSCLC cells were explored by CCK-8, Transwell invasion assay, flow cytometry analysis and caspase-3/7 activity. AOC4P was lowly expressed in NSCLC samples and cells. Overexpression of AOC4P inhibited viability, the expression of MMP-2 and MMP-9, and invasion of NSCLC cells. Apoptosis and caspase-3/7 activity were suppressed in response to AOC4P overexpression in NSCLC cells. AOC4P overexpression suppressed tumor growth in a xenograft mouse model. A ctivation of the Wnt/β-catenin pathway by BML-284 abolished the effects of AOC4P overexpression on cell viability, invasion and apoptosis in NSCLC cells. In conclusion, AOC4P overexpression suppresses viability and invasion and induces apoptosis in NSCLC cells via inhibition of the Wnt/β-catenin pathway. Highlights: AOC4P was lowly expressed in NSCLC cells. AOC4P overexpression inhibited the proliferation and the Wnt/β-catenin pathway in NSCLC cells. AOC4P overexpression suppressed the invasion and induced apoptosis of NSCLC cells. AOC4P overexpression suppressed tumor growth in a xenograft mouse model.