Dual-crosslinked methylcellulose hydrogels for 3D bioprinting applications. (15th June 2020)

• Record Type: Journal Article
• Authors: Shin, Ji Youn; Yeo, Yong Ho; Jeong, Jae Eun; Park, Su A.; Park, Won Ho
• Is Part Of: Carbohydrate polymers. Volume 238(2020)
• Publication Date: 2020-06-15
• Subjects: 3D bioprinting -- bioink -- dual crosslinkable hydrogel -- methylcellulose -- tyramine -- riboflavin 5'-monophosphate; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2020.116192
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: Dual crosslinkable tyramine-modified methylcellulose (MC-Tyr) was prepared via two-step synthesis. MC-Tyr hydrogel showed excellent mechanical properties and printability for 3D bioprinting. Dual-crosslinked MC-Tyr hydrogel has a great potential as a novel bioink for 3D bioprinting. Abstract: Thermo-sensitive methylcellulose (MC) hydrogel has been widely used as a scaffold material for biomedical applications. However, due to its poor mechanical properties, the MC-based hydrogel has rarely been employed in 3D bioprinting for tissue engineering scaffolds. In this study, the dual crosslinkable tyramine-modified MC (MC-Tyr) conjugate was prepared via a two-step synthesis, and its hydrogel showed excellent mechanical properties and printability for 3D bioprinting applications. The MC-Tyr conjugate formed a dual-crosslinked hydrogel by modulating the temperature and/or visible light. A combination of reversible physical crosslinking (thermal crosslinking) and irreversible chemical crosslinking (photocrosslinking) was used in this dual crosslinked hydrogel. Also, the photocrosslinking of MC-Tyr solution was facilitated by visible light exposure in the presence of biocompatible photoinitiators (riboflavin, RF and riboflavin 5'-monophophate, RFp). The RF and RFp were used to compare the cytotoxicity and salting-out effect of MC-Tyr hydrogel, as well as the initiation ability, based on the difference in chemical structure. Also, the influence of the printing parameters on the printed MC hydrogel was investigated. Finally, the cell-laden MC-Tyr bioink was successfully extruded into stable 3D hydrogel constructs with high resolution via a dual crosslinking strategy. Furthermore, the MC-Tyr scaffolds showed excellent cell viability and proliferation.