First detection of multiresistance pRE25-like elements from Enterococcus spp. in Staphylococcus pseudintermedius isolated from canine pyoderma. (March 2020)

• Record Type: Journal Article
• Authors: Kang, Jung-Hun; Hwang, Cheol-Yong
• Is Part Of: Journal of global antimicrobial resistance. Volume 20(2020)
• Page Start: 304
• Page End: 308
• Publication Date: 2020-03
• Subjects: Multidrug resistance -- pRE25-like elements -- Staphylococcus pseudintermedius; Drug resistance -- Periodicals; Drug resistance -- Periodicals; Drug resistance; Periodicals; 616.9041
• DOI: 10.1016/j.jgar.2019.08.022
• Languages: English
• ISSNs: 2213-7165

Abstract

Highlights: Chromosomal pRE25-like elements carrying five antimicrobial resistant genes ( cat, erm (B), aphA3, aadK, and sat4 ) were first detected in Staphylococcus spp. The 50-kb enterococcal pRE25 genome covered 84% of the 22-kb pRE25-like elements sequence, with 99.8% percent identity. Multiresistance pRE25-like elements are suspected to be widespread in Staphylococcus pseudintemedius isolated from companion dogs in Korea. The constant investigation about transferring to the staphylococci commonly isolated from humans (e.g., S . aureus and S . epidermidis ) is required. Abstract: Objectives: Enterococcus pRE25 is a conjugative and mobilising multiresistance plasmid from Enterococcus faecalis RE25. pRE25-like enterococcal plasmid pWZ909 mediates the delivery of vancomycin resistance to methicillin-resistant Staphylococcus aureus via a Tn 1546 -like transposon. However, whether pRE25-like elements contribute to multidrug resistance in Staphylococcus spp. has not yet been investigated. Here we describe the first detection of multiresistance pRE25-like elements in the chromosomal DNA of multidrug-resistant Staphylococcus pseudintermedius (MDRSP). Methods: A total of 46 MDRSP clinical strains were isolated from canine pyoderma in Korea. Their genetic characteristics were analysed by multilocus sequence typing (MLST) and PCR targeting pRE25-like elements. Whole-genome sequencing (WGS) was performed on four isolates. Results: WGS detected that the chromosomal 22-kb pRE25-like elements contained five antimicrobial resistance genes [ cat, erm (B), aphA-3, aadK and sat4 ], IS 1252, IS 256, and a toxin–antitoxin system within copies of IS 1216. BLASTn alignment analysis revealed that 84% of the chromosomal 22-kb pRE25-like elements sequence is homologous (99.8% identity) to the enterococcal pRE25 plasmid sequence. PCR assay showed that 52.2% of MDRSP isolates were positive for pRE25-like elements and were presumed to contain pRE25-like elements (pRE25 group). The sequence types (STs) of the pRE25 group were diverse, with 18 STs identified, among which 12 STs were first reported in Korea. Conclusion: Enterococcal pRE25-like elements are suspected to be widespread in MDRSP isolated from companion dogs in Korea. Considering that companion dogs live in a closely shared environment with humans, continuous surveillance of pRE25-like elements is needed for other staphylococci commonly isolated from humans.