Bioallethrin-induced generation of reactive species and oxidative damage in isolated human erythrocytes. (June 2020)

• Record Type: Journal Article
• Authors: Arif, Amin; Salam, Samreen; Mahmood, Riaz
• Is Part Of: Toxicology in vitro. Volume 65(2020)
• Publication Date: 2020-06
• Subjects: Bioallethrin -- Erythrocytes -- Antioxidant power -- Oxidative stress -- Methemoglobin -- ROS; AFR ascorbate free radical -- AO antioxidant -- AOPP advanced oxidation protein products -- CUPRAC cupric reducing antioxidant capacity -- DHE dihydroethidium -- DPPH 2, 2-diphenyl-1-picrylhydrazyl -- FR free radicals -- FRAP ferric reducing/antioxidant power -- GSH reduced glutathione -- GSSG oxidised glutathione -- Hb hemoglobin -- MetHb methemoglobin -- PMA phosphomolybdenum assay -- PMRS plasma membrane redox system -- RNS reactive nitrogen species -- ROS reactive oxygen species; Toxicity testing -- In vitro -- Periodicals; Toxicology -- Periodicals; 615.9
• DOI: 10.1016/j.tiv.2020.104810
• Languages: English
• ISSNs: 0887-2333

Abstract

Abstract: Bioallethrin is an insecticide that is widely used to control mosquitoes, fleas and cockroaches. The widespread use of bioallethrin has resulted in both occupational and non-occupational human exposure. Bioallethrin enters blood, regardless of the route of exposure, where it can interact with erythrocytes. We have studied the effect of bioallethrin on isolated human erythrocytes under in vitro conditions. Erythrocytes were incubated with increasing concentrations of bioallethrin (10–200 μM) for 4 h at 37 °C. Several biochemical parameters were analyzed in bioallethrin treated and untreated (control) cells. Incubation of erythrocytes with bioallethrin increased protein oxidation, lipid peroxidation and depleted sulfhydryl group content. Membrane damage was evident from cell lysis, osmotic fragility, inhibition of bound enzymes and transmembrane electron transport system. Bioallethrin also increased hemoglobin oxidation, heme degradation and the release of free iron moiety. This will decrease the oxygen transporting ability of blood. Bioallethrin treatment altered the specific activities of antioxidant enzymes and diminished the antioxidant power of cells. Scanning electron microscopy showed that bioallethrin treatment also altered erythrocyte mophology. Almost all changes were in a bioallethrin concentration dependent manner. The cytotoxicity of bioallethrin is probably mediated by reactive oxygen and nitrogen species whose formation was significantly enhanced in treated erythrocytes. Thus bioallethrin enhances the generation of reactive species which cause oxidative damage of cell components in human erythrocytes. Graphical abstract: Unlabelled Image Highlights: Bioallethrin enhances the generation of ROS, RNS in human erythrocytes. The cellular enzymatic and non-enzymatic defence is impaired. Hemoglobin is oxidised to methemogloin that is inactive in oxygen transport. Bioallethrin damages plasma membrane and changes erythrocyte morphology. Adverse effects of bioallethrin on erythrocytes can cause anemia in humans.