Gelatin Matrices for Growth Factor Sequestration. Issue 5 (May 2020)

• Record Type: Journal Article
• Authors: Buie, Taneidra; McCune, Joshua; Cosgriff-Hernandez, Elizabeth
• Is Part Of: Trends in biotechnology. Volume 38:Issue 5(2020)
• Page Start: 546
• Page End: 557
• Publication Date: 2020-05
• Subjects: gelatin -- growth factor -- controlled release -- sequestration -- affinity; Biotechnology -- Periodicals; Biochemical engineering -- Periodicals; Genetic engineering -- Periodicals; Industrial microbiology -- Periodicals; 660.605
• DOI: 10.1016/j.tibtech.2019.12.005
• Languages: English
• ISSNs: 0167-7799

Abstract

Abstract : Gelatin is used in a broad range of tissue engineering applications because of its bioactivity, mild processing conditions, and ease of modification, which have increased interest in its use as a growth factor delivery vehicle. Traditional methods to control growth factor sequestration and delivery have relied on controlling hydrogel mesh size via chemical crosslinking with corollary changes to the physical properties of the hydrogel. To decouple growth factor release from scaffold properties, affinity sequestration modalities have been developed to preserve the bioactivity of the growth factor through interactions with the modified gelatin. This review provides a summary of these mechanisms, highlights current gelatin growth factor delivery systems, and addresses the future perspective of gelatin matrices for growth factor delivery in tissue engineering. Highlights: Traditional methods of growth factor sequestration and delivery by gelatin matrices have relied on controlling hydrogel mesh size via chemical crosslinking with corollary changes to the physical properties of the hydrogel. Growth factors are generally not directly conjugated to gelatin matrices because of potential loss of bioactivity owing to non-specific reactions with amino groups. Elucidating non-covalent growth factor interactions has led to the development of affinity-based methods to increase sequestration in gelatin matrices. Modifying gelatin matrices to confer targeted growth factor affinity has potential to improve tissue regeneration over traditional crosslinking methods.