Time- and compound-dependent microbial community compositions and oil hydrocarbon degrading activities in seawater near the Chinese Zhoushan Archipelago. (March 2020)

• Record Type: Journal Article
• Authors: Peng, Chao; Tang, Yun; Yang, Hong; He, Yan; Liu, Yan; Liu, Dan; Qian, Yongming; Lu, Lu
• Is Part Of: Marine pollution bulletin. Volume 152(2020)
• Publication Date: 2020-03
• Subjects: Marine oil spill -- Hydrocarbon biodegradation -- Oil-degrading bacteria -- Radiotracer assay -- Bacterial activity -- Zhoushan archipelago; Marine pollution -- Periodicals; Marine Biology -- Periodicals; Water Pollution -- Periodicals; Mer -- Pollution -- Périodiques; Publications périodiques; Pollution des mers; Lutte antipollution; Electronic journals; 363.7394
• DOI: 10.1016/j.marpolbul.2020.110907
• Languages: English
• ISSNs: 0025-326X

Abstract

Abstract: Marine microorganisms play an irreplaceable role in removing spilled oil. Zhoushan archipelago has one of the busiest ports and oil stockpiles in China. However, little is known about which and how fast oil-degrading microorganisms could biodegrade spilled oil here. By combining 14 C-/ 3 H-based radiotracer assays and MiSeq sequencing, we report the successive pattern of microbial oil-degrading activities and community compositions. The biodegradation rates of alkanes and PAHs (Polycyclic Aromatic Hydrocarbons) were significantly stimulated by oil addition, and reached their maximum after incubation for 3 and 7 days, respectively. Meanwhile, the abundances of alkB and phnA c genes increased and the bacterial communities continuously shifted. Potential oil-degrading bacteria Alcanivorax, Erythrobacter were the dominant degraders by day 3, whereas the dominant degraders shifted to C1-B045, Alteromonas, Pseudohongiella in the later period. These results provide valuable insights into the cooperative system of the versatile oil-degrading bacteria in successively biodegrading complex oil hydrocarbons in oil spills. Highlights: Biodegradation of alkanes and PAHs were time-dependent in marine oil spills. Oil spill significantly increased the abundance of 16S rRNA, alkB, phnAc genes. Highly active oil biodegradation was developed within hours right after oil spill. The dominant oil-degrading bacteria varied as time progressed.