Identification of heme oxygenase-1 from golden pompano (Trachinotus ovatus) and response of Nrf2/HO-1 signaling pathway to copper-induced oxidative stress. (August 2020)

• Record Type: Journal Article
• Authors: Xie, Jiajun; He, Xuanshu; Fang, Haohang; Liao, Shiyu; Liu, Yongjian; Tian, Lixia; Niu, Jin
• Is Part Of: Chemosphere. Volume 253(2020)
• Publication Date: 2020-08
• Subjects: HO-1 -- RACE -- Nrf2/HO-1 signaling pathway -- Trachinotus ovatus; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2020.126654
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: Heme oxygenase-1(HO-1) is a stress-inducible enzyme that mediates antioxidative and cytoprotective effects to maintain cellular redox homeostasis. In the present study, the full sequence of HO-1 was cloned from golden pompano( Trachinotus ovatus ) by RT-PCR and RACE-PCR. The full cDNA sequence of HO-1 was 1349 bp in length which comprised of a 726 bp open reading frame (ORF) preceded by 262 bp 5′-untranslated region (UTR), and followed by a 360 bp 3′UTR, encoding 241 amino acid residues. Phylogenetic analysis revealed that HO-1 showed highest similarity to that of Takifugu rubripes . Tissue distribution analysis showed that the expression level of HO-1 was relatively high in heart, liver and spleen. A trial was conducted to investigate the response of Nrf2/HO-1 signaling pathway to oxidative stress induced by copper. The results showed that mRNA expression of NF-E2-related nuclear factor2 (Nrf2), Kelch-like-ECH-associated protein1 (keap1), superoxide dismutase (SOD), catalase (CAT), HO-1, NAD(P)H quinone oxidoreductase 1 (NQO1) and Glutathione peroxidase (GSH-PX) all significantly increased in copper treated group than that in the control group. This work provides new insight into the molecular mechanism underlying the Nrf2/HO-1 pathway in oxidative response in T. ovatus . Highlights: HO-1 cDNA was cloned from golden pompano, Trachinotus ovatus. HO-1 mRNA was highly expressed in heart. The Nrf2/HO-1 pathway was up-regulated by copper-induced oxidative stress.