Adenosylation reactions catalyzed by the radical S-adenosylmethionine superfamily enzymes. (April 2020)

• Record Type: Journal Article
• Authors: Ding, Wei; Ji, Xinjian; Zhong, Yuting; Xu, Kuang; Zhang, Qi
• Is Part Of: Current opinion in chemical biology. Volume 55(2020)
• Page Start: 86
• Page End: 95
• Publication Date: 2020-04
• Subjects: Radical addition -- Biosynthesis -- Natural product -- Nucleoside -- Biocatalysis; Bioorganic chemistry -- Periodicals; Biology -- Periodicals; Biochemistry -- Periodicals; Clinical biochemistry -- Periodicals; Biochemistry -- Periodicals; Chimie bio-organique -- Périodiques; Biologie -- Périodiques; 572.05
• DOI: 10.1016/j.cbpa.2020.01.007
• Languages: English
• ISSNs: 1367-5931

Abstract

Abstract: The radical S-adenosylmethionine (SAM) superfamily enzymes reductively cleave SAM to produce a highly reactive 5ˊ-deoxyadenosyl (dAdo) radical, which in most cases abstracts a hydrogen from the substrate and initiates highly diverse reactions. In rare cases, the dAdo radical can add to a sp 2 carbon to result in the production an adenosylated product. These radical SAM-dependent adenosylation reactions are present in natural product biosynthetic pathways and can be achieved by using unnatural substrate analogs containing olefin or aryl moieties. This Opinion provides a focused perspective on this emerging type of biochemistry and discusses its potential use in bioengineering and biocatalysis.