A Siglec-1-like lectin from Nile tilapia (Oreochromis niloticus) possesses functions of agglutination and mediation of macrophage phagocytic activity. Issue 102 (July 2020)

• Record Type: Journal Article
• Authors: Kong, Linghe; Wu, Liting; Guo, Zheng; Mu, Liangliang; Yang, Yanjian; Bian, Xia; Li, Bingxi; Pan, Xunbin; Fu, Shengli; Ye, Jianmin
• Is Part Of: Fish & shellfish immunology. Issue 102(2020)
• Page Start: 203
• Page End: 210
• Publication Date: 2020-07
• Subjects: Oreochromis niloticus -- Siglec-1-like -- Streptococcus agalactiae -- Agglutination -- Phagocytosis; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2020.04.034
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Siglec-1, one of the sialic acid-binding immunoglobulin-type lectins, is closely related to the recognition of host-pathogen and cell-cell interactions in the adaptive and innate immune systems. In this communication, a Siglec-1 -like gene ( OnSiglec-1 -like) from Nile tilapia ( Oreochromis niloticus ) was analyzed. Relative expression revealed that the OnSiglec-1 -like was expressed in all tested tissues, and the highest expression was found in the anterior kidney. Upon Streptococcus agalactiae ( S. agalactiae ) infection, the expression of OnSiglec-1 -like was up-regulated in anterior kidney and spleen significantly in vivo . Additionally, the same phenomenon was observed in anterior kidney leukocytes upon LPS and S. agalactiae challenges as well in vitro . Western-blotting and ELISA analyses revealed that recombinant OnSiglec-1-like protein possessed high binding activity to LTA, LPS and S. agalactiae . Further, the recombinant OnSiglec-1-like was able to agglutinate S. agalactiae . Moreover, with the digestion of specific sialidase, the phagocytic ability of macrophages to S. agalactiae was greatly enhanced. Taken together, these results indicated that the Siglec-1-like possesses conserved functions of agglutination and promotion of macrophage phagocytic activity in Nile tilapia. Highlights: The expression of OnSiglec-1 -like was significantly up-regulated after challenge with Streptococcus agalactiae ( in vivo and in vitro ). Recombinant OnSiglec-1-like was able to combine and agglutinate Streptococcus agalactiae. OnSiglec-1-like could recognize Streptococcus agalactiae and mediate macrophage phagocytosis of bacteria. OnSiglec-1-like could get involved in response against Streptococcus agalactiae infection in Nile tilapia.