Transcriptome and metabolome integration analysis of mud crab Scylla paramamosain challenged to Vibrio parahaemolyticus infection. Issue 103 (August 2020)

• Record Type: Journal Article
• Authors: Kong, Tongtong; Lin, Shanmeng; Ren, Xin; Li, Shengkang; Gong, Yi
• Is Part Of: Fish & shellfish immunology. Issue 103(2020)
• Page Start: 430
• Page End: 437
• Publication Date: 2020-08
• Subjects: Vibrio parahaemolyticus -- Scylla paramamosain -- Metabolome -- Transcriptome; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2020.05.069
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Vibrio parahaemolyticus ( V. parahaemolyticus ) is a common pathogen for marine crustacean, which causes severe illnesses in aquatic animals. Therefore, it is meaningful to explore the mechanism during V. parahaemolyticus infection. In this study, to investigate the immune responses of mud crab Scylla paramamosain ( S. paramamosain ) to V. parahaemolyticus, we established the metabolic and transcriptional profiles of mud crab hemocytes challenged with V. parahaemolyticus . The results indicated that V. parahaemolyticus infection could induce a series of metabolism alterations at both metabolome and transcriptome levels, including biosynthesis of amino acids and Aminoacyl-tRNA, Purine and pyrimidine metabolism, TCA cycle and glutamine metabolism. In this context, through the integration of metabolomics and transcriptomics, our study provided a more comprehensive understanding of the biological process in mud crab against pathogen infection. Highlights: Metabolic and transcriptional profiles in mud crab challenged with V. p were integrated analyzed. V. p infection could induce a series of metabolism alterations at both metabolome and transcriptome levels in mud crab. The altered metabolic processes include biosynthesis of amino acids, purine and pyrimidine metabolism and so on.