Molecular characterisation of ILRUN, a novel inhibitor of proinflammatory and antimicrobial cytokines. Issue 6 (June 2020)

• Record Type: Journal Article
• Authors: Ambrose, Rebecca L.; Brice, Aaron M.; Caputo, Alessandro T.; Alexander, Marina R.; Tribolet, Leon; Liu, Yu Chih; Adams, Timothy E.; Bean, Andrew G.D.; Stewart, Cameron R.
• Is Part Of: Heliyon. Volume 6:Issue 6(2020)
• Publication Date: 2020-06
• Subjects: Immunolgy -- Inflammation -- Infectious disease -- Immune response -- Viruses -- Biochemistry -- Biomolecules -- ILRUN -- C6orf106 -- Innate immunity -- Interferon -- p300 -- CBP; Research -- Periodicals; Medical sciences -- Periodicals; Natural history -- Periodicals; Social sciences -- Periodicals; Earth sciences -- Periodicals; Physical sciences -- Periodicals; 507.2
• DOI: 10.1016/j.heliyon.2020.e04115
• Languages: English
• ISSNs: 2405-8440

Abstract

Abstract: Regulation of type-I interferon (IFN) production is essential to the balance between antimicrobial defence and autoimmune disorders. The human protein-coding gene ILRUN (inflammation and lipid regulator with UBA-like and NBR1-like domains, previously C6orf106) was recently characterised as an inhibitor of antiviral and proinflammatory cytokine (interferon-alpha/beta and tumor necrosis factor alpha) transcription. Currently there is a paucity of information about the molecular characteristics of ILRUN, despite it being associated with several diseases including virus infection, coronary artery disease, obesity and cancer. Here, we characterise ILRUN as a highly phylogenetically conserved protein containing UBA-like and a NBR1-like domains that are both essential for inhibition of type-I interferon and tumor necrosis factor alpha) transcription in human cells. We also solved the crystal structure of the NBR1-like domain, providing insights into its potential role in ILRUN function. This study provides critical information for future investigations into the role of ILRUN in health and disease. Abstract : Immunolgy; Inflammation; Infectious disease; Immune Response; Viruses; Biochemistry; Biomolecules; ILRUN; C6orf106; innate immunity; interferon; p300; CBP