Characterization and transcriptomic analysis of a highly Cr(VI)-resistant and -reductive plant-growth-promoting rhizobacterium Stenotrophomonas rhizophila DSM14405T. (August 2020)

• Record Type: Journal Article
• Authors: Gao, Jie; Wu, Shimin; Liu, Ying; Wu, Shanghua; Jiang, Cancan; Li, Xianglong; Wang, Rui; Bai, Zhihui; Zhuang, Guoqiang; Zhuang, Xuliang
• Is Part Of: Environmental pollution. Volume 263(2020)Supplement Part B
• Publication Date: 2020-08
• Subjects: Stenotrophomonas rhizophila -- Cr(VI) -- Resistance -- Reduction -- Transcriptomic analysis; Pollution -- Periodicals; Pollution -- Environmental aspects -- Periodicals; Environmental Pollution -- Periodicals; Pollution -- Périodiques; Pollution -- Aspect de l'environnement -- Périodiques; Pollution -- Effets physiologiques -- Périodiques; Pollution; Pollution -- Environmental aspects; Periodicals; Electronic journals; 363.73
• DOI: 10.1016/j.envpol.2020.114622
• Languages: English
• ISSNs: 0269-7491

Abstract

Abstract: Previous research has shown that Stenotrophomonas has the ability to reduce Cr(VI). In this study, we determined whether the reduction capacity of Cr(VI) is conserved in Stenotrophomonas rhizophila DSM14405 T, a plant-growth-promoting rhizobacterium (PGPR). Our results show that S. rhizophila DSM14405 T displays high Cr(VI) resistance at a minimal inhibitory concentration of 1000 mg/L. Furthermore, it completely reduced 50 mg/L Cr(VI) in 28 h at pH 7.5 at 30 °C. The results of X-ray photoelectron spectroscopy and high performance liquid chromatography coupled to inductively coupled plasma mass spectrometry analysis confirmed the ability of S. rhizophila DSM14405 T to convert Cr(VI) to Cr(III), and indicated the adsorption and intracellular accumulation of Cr(III). Transcriptomic analysis revealed that in the presence or absence of Cr(VI), transcriptomes upon short-term exposure showed more differentially expressed genes than those after long-term exposure. GO and KEGG analyses showed that most of the differentially expressed genes were related to Cr(VI) resistance, including genes related to iron homeostasis, central metabolism, DNA repair and anti-oxidative stress, and sulfur metabolism. Highly Cr(VI)-resistant and reductive abilities of this PGPR strain render it a suitable candidate for combined plant-microbe remediation of chromium contaminants from soil. Graphical abstract: Image 1 Highlights: A PGPR Stenotrophomonas rhizophila, displays high Cr(VI) resistance and reductive ability. S. rhizophila can completely reduce 50 mg/L Cr(VI) within 28 h. High salt has less stress on S. rhizophila Cr(VI) reduction efficiency. The mechanism underlying Cr(VI) resistance and reduction of S. rhizophila was revealed. Results provide new candidate for combined plant-microbe remediation of Cr(VI). Abstract : Mechanism underlying Cr(VI) resistance and reduction of a plant-growth-promoting rhizobacterium - Stenotrophomonas rhizophila .