MeCP2 mediates transgenerational transmission of chronic pain. (June 2020)

• Record Type: Journal Article
• Authors: Tao, Wenjuan; Chen, Changmao; Wang, Yuping; Zhou, Wenjie; Jin, Yan; Mao, Yu; Wang, Haitao; Wang, Likui; Xie, Wen; Zhang, Xulai; Li, Jie; Li, Juan; Li, Xiangyao; Tang, Zhen-Quan; Zhou, Chenghua; Pan, Zhizhong Z.; Zhang, Zhi
• Is Part Of: Progress in neurobiology. Volume 189(2020)
• Publication Date: 2020-06
• Subjects: Chronic pain -- MeCP2 -- Primary somatosensory cortex -- Glutamatergic neuron -- Transgenerational transmission; Neurobiology -- Periodicals; Neurology -- Periodicals; Neurology -- Periodicals; Neurobiologie -- Périodiques; 612.8
• DOI: 10.1016/j.pneurobio.2020.101790
• Languages: English
• ISSNs: 0301-0082

Abstract

Highlights: The female offspring from maternal mice with pain displayed pain sensitization. MeCP2 mediates pain transgenerational transmission in female mice. MeCP2 regulates S1 glutamatergic neuronal activity. MeCP2 regulates gene expression in S1 glutamatergic neurons in pain states. Abstract: Pain symptoms can be transmitted across generations, but the mechanisms underlying these outcomes remain poorly understood. Here, we identified an essential role for primary somatosensory cortical (S1) glutamate neuronal DNA methyl-CpG binding protein 2 (MeCP2) in the transgenerational transmission of pain. In a female mouse chronic pain model, the offspring displayed significant pain sensitization. In these mice, MeCP2 expression was increased in S1 glutamate (Glu S1 ) neurons, correlating with increased neuronal activity. Downregulation of Glu S1 neuronal MeCP2 in maternal mice with pain abolished offspring pain sensitization, whereas overexpression of MeCP2 in naïve maternal mice induced pain sensitization in offspring. Notably, single-cell sequencing and chromatin immunoprecipitation analysis showed that the expression of a wide range of genes was changed in offspring and maternal Glu S1 neurons, some of which were regulated by MeCP2. These results collectively demonstrate the putative importance of MeCP2 as a key regulator in pain transgenerational transmission through actions on Glu S1 neuronal maladaptation.