Influence of dietary iron exposure on trace metal homeostasis and expression of metal transporters during development in zebrafish☆. (June 2020)

• Record Type: Journal Article
• Authors: Chandrapalan, Theanuga; Kwong, Raymond W.M.
• Is Part Of: Environmental pollution. Volume 261(2020)
• Publication Date: 2020-06
• Subjects: Metal homeostasis -- Iron -- Dietary exposure -- Zebrafish -- Development; Pollution -- Periodicals; Pollution -- Environmental aspects -- Periodicals; Environmental Pollution -- Periodicals; Pollution -- Périodiques; Pollution -- Aspect de l'environnement -- Périodiques; Pollution -- Effets physiologiques -- Périodiques; Pollution; Pollution -- Environmental aspects; Periodicals; Electronic journals; 363.73
• DOI: 10.1016/j.envpol.2020.114159
• Languages: English
• ISSNs: 0269-7491

Abstract

Abstract: The present study investigated the effects of dietary iron (Fe) exposure on physiological performance and homeostatic regulation of trace metals during development (5–28 days post-fertilization; dpf) in zebrafish ( Danio rerio ). The results demonstrated that whole body Fe content was increased in 14 dpf larvae fed a high Fe diet. Cumulative mortality was also significantly elevated during exposure to the high Fe diet. Using droplet digital PCR, we observed that high Fe-exposed larvae exhibited an increase in mRNA levels of the Fe-storage protein ferritin, which appeared to be associated with the elevated level of whole body Fe content. Further, the results indicated that dietary Fe exposure induced transient changes in the mRNA expression levels of various metal transporters, including the iron transporter dmt1, and the zinc transporters zip8 and zip14 . The expression of the epithelial Ca 2+ channels (i.e., ecac ) was also found to increase by high dietary Fe. Overall, our findings suggest that larval fish during the early nutritional transition period are sensitive to the effects of dietary Fe. Graphical abstract: Image 1 Highlights: Fish during the early nutritional transition stages are sensitive to dietary Fe. Dietary Fe exposure leads to dysregulation in Fe balance and increased mortality. Several metal-regulatory genes are modulated by dietary Fe during development. Abstract : Dietary iron exposure affects metal regulation in developing zebrafish.