Antibacterial and antibiofilm activities of diphyllin against fish pathogens. (August 2020)

• Record Type: Journal Article
• Authors: Thamburaj, Suman; Ramaraj, Elangomathavan; Sethupathy, Sivasamy; Kamalanathan, Chakkaravarthi; Raji, Atchudan; Rajasekharan, Satish Kumar
• Is Part Of: Microbial pathogenesis. Volume 145(2020)
• Publication Date: 2020-08
• Subjects: Diphyllin -- Antibacterial -- MIC -- MBC -- Biofilm -- DNA damage -- ROS; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2020.104232
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: Annihilation of biofilm forming bacterial pathogens is a challenging aspect in seafood and aquaculture industries. Microbes growing as biofilms cause deleterious effects on food products leading to food spoilage or loss of shelf life. As a measure to fight biofilms, agents that prevent/disrupt biofilms are recurrently screened. The study exemplifies the bactericidal and biofilm disruption potentials of a plant derived compound, diphyllin, against fish pathogens that colonizes Oreochromis mossambicus and Oreochromis niloticus. Precisely, diphyllin disrupted Salmonella typhi biofilms by triggering reactive oxidative species (ROS). Diphyllin-induced ROS had satisfactory correlation with S. typhi cell membrane damage and intracellular DNA degradation profiles providing a putative mechanistic model. In conclusion, the study identifies diphyllin as a therapeutic and dispersal agent aimed at biofilms formed by food-borne pathogens that persistently plague food processing and aquaculture settings. Highlights: Diphyllin exhibited potent bactericidal effect against several fish pathogens. Diphyllin prevented/disrupted Salmonella typhi biofilms. Diphyllin treatment also resulted in DNA and membrane damage. Overall, diphyllin seemed to achieve bacterial killing by ROS induction.