Macrophage-stimulating activities of a novel low molecular weight saccharide fragment prepared from ascophyllan with alginate lyase. (April 2020)

• Record Type: Journal Article
• Authors: Jiang, Zedong; Yu, Gang; Bao, Qingyun; Xu, Xu; Zhu, Yanbing; Ni, Hui; Li, Qingbiao; Oda, Tatsuya
• Is Part Of: Journal of functional foods. Volume 67(2020)
• Publication Date: 2020-04
• Subjects: Ascophyllum nodosum -- Ascophyllan -- Low-molecular-weight saccharide -- Structural characteristic -- Macrophage-stimulating activity -- Intracellular signal pathway; ELISA enzyme linked immunosorbent assay -- TNF-α mouse tumor necrosis factor-alpha -- ROS reactive oxygen species -- PDTC ammonium pyrrolidinedithiocarbamate -- NF-κB nuclear factor-kappa B -- MAP kinase mitogen-activated protein kinase -- JNK c-jun N-terminal kinase -- ERK extracellular-regulated kinase -- PBS phosphate buffer saline -- HPSEC high performance size-exclusion chromatography -- NO nitric oxide -- DCFH-DA 2′, 7′-dichlorodihydrofluorescein diacetate -- FT-IR fourier transform infrared spectra -- KBr potassium bromide -- PMAA partially methylated alditol acetates -- NMR nuclear magnetic resonance -- HSQC heteronuclear single quantum coherence -- HMBC heteronuclear multiple bond correlation -- COSY correlation spectroscopy -- Fucp L-fucopyranose -- Manp D-mannopyranose -- ManpA D-mannopyranosyluronic acid -- Xylp D-xylopyranose -- Galp D-galactopyranose -- Glcp D-glucopyranose -- GlcpA D-glucopyranosyluronic acid; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2020.103839
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: Sulphated polysaccharide ascophyllan can be degraded by alginate lyase, EC 4.2.2.3. A novel low molecular mass fragment LMWAs-L is purified from enzymolysis products. LMWAs-L is a low fucose- and sulphate-containing saccharide fragment. LMWAs-L exhibits significant macrophage-stimulating activities in vitro . The proposal structure of LMWAs-L was established. Abstract: In this study, the polysaccharide ascophyllan, isolated from Ascophyllum nodosum, was degraded using alginate lyase (EC 4.2.2.3) to prepare low-molecular-weight fragments. LMWAs-L, an extremely low-molecular-weight saccharide (6.71 kDa) with low fucose- and sulphate-contents, was purified by gel filtration chromatography. LMWAs-L exhibited significant macrophage-stimulating activities, similar to ascophyllan, through intracellular signalling pathways. Methylation and GC/MS analyses indicated that the main glycosidic linkages of LMWAs-L were 1, 2-linked-Glc p A, followed by T -linked-Glc p, 1, 3, 6-linked-Gal p, 1, 3-linked-Fuc p, 1, 4-linked-Xyl p, and 1, 4-linked-Man p A at a molar ratio of 3.37: 1.13: 1.11: 1.00: 0.96: 0.61. The proposal repeating structure of LMWAs-L was shown to be →2)-α-D-Glc p A-(1 → 2)-α-D-Glc p A-(1 → 6)-α-D-Gal p -(1 → 2)-α-D-Glc p A-(1→ as main chain, branched by T -α-D-Glc p -(1 → 4)-β-D-Xyl p -(1 → 3)-α-L-Fuc p 4S-(1→ at the O -3 of 3, 6)-α-D-Gal p -(1→ residues. 4-deoxy-L- erythro -hex-4-enuronosyluronate-4-β-D-Man p A-(1→ and →4)-β-D-Man p A red residues were attached to the ends of main chain as non-reducing end residue and reducing end residue, respectively. Our results suggest that the fragment LMWAs-L contain an essential structural element of ascophyllan that is responsible for the macrophage-stimulating activities.