Shift in bacterial diversity in acidogenesis of gelatin and gluten seeded with various anaerobic digester inocula. (June 2020)

• Record Type: Journal Article
• Authors: Kim, Su In; Kim, Eunji; Aghasa, Aghasa; Hwang, Seokhwan
• Is Part Of: Bioresource technology. Volume 306(2020)
• Publication Date: 2020-06
• Subjects: Anaerobic digestion -- Food industrial wastewater -- Animal protein -- Plant protein -- Bacterial community; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2020.123158
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Various anaerobic seeds were tested for gelatin and gluten degradation. Clostridium, Peptoclostridium, Hathewaya were dominant genera in gelatin degradation. Klebsiella was a dominant genus in gluten degradation. The highest rate coefficient was estimated in gelatin degradation and was 2.3 (d −1 ). Abstract: The aim of this study was to investigate divergence of bacteria degrading model proteins of food-processing wastewater. Gelatin and gluten were used as substrate to represent animal and plant proteins from food wastes, respectively. The inocula were obtained from eight full-scale anaerobic digestion reactors. Food-to-microorganism ratio was 3 g chemical oxygen demand equivalent of substrate per 1 g volatile suspended solids of inoculum. A first-order reaction model revealed reaction constants ranged 1.34 ≤ k ≤ 2.30 d −1 for gelatin and 0.63 ≤ k ≤ 1.69 d −1 for gluten. Metagenomic analysis of 16s rRNA sequences showed that dominant bacteria after gelatin degradation batch were different for each inocula. Klebsiella aerogenes, Hathewaya, Peptoclostridium, or Clostridium collagenovorans were most abundant. Klebsiella aerogenes was the most abundant species after gluten degradation for all inocula.