Differential effects of inulin or its fermentation metabolites on gut barrier and immune function of porcine intestinal epithelial cells. (April 2020)

• Record Type: Journal Article
• Authors: Uerlings, Julie; Schroyen, Martine; Willems, Els; Tanghe, Sofie; Bruggeman, Geert; Bindelle, Jérôme; Everaert, Nadia
• Is Part Of: Journal of functional foods. Volume 67(2020)
• Publication Date: 2020-04
• Subjects: Prebiotic -- In vitro fermentation -- Porcine intestinal epithelial cells -- Short-chain fatty acids -- Gut barrier -- Inflammatory response; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2020.103855
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: Inulin fermentation in the gut leads to the production of beneficial metabolites. The fermentation model was combined with pig intestinal epithelial cells (IPEC-J2). Inulin was associated with elevated butyrate levels. Inulin fermentation products enhanced gut barrier function in IPEC-J2 cells. The increase in butyrate could account for inulin bioactive property. Abstract: Prebiotics can modulate gut fermentation and improve intestinal barrier function in mammals. First, inulin fermentation profile was tested in a three-step in vitro model of the piglet's gastro-intestinal tract combining a hydrolysis – dialysis step to a batch fermentation. Then, the differential effects of digested inulin (after the hydrolysis – dialysis steps) or fermented inulin (after the fermentation step) on the expression of gut barrier and immune-related genes of IPEC-J2 cells were investigated by high-throughput qPCR. Inulin was associated with elevated short-chain fatty acids and butyrate levels. Upregulated expressions of tight and adherens junction genes were observed in IPEC-J2 cells supplemented with inulin fermentation supernatant compared to control IPEC-J2 cells and digested inulin. Therefore, metabolites arising from the fermentation process, including butyrate, could be responsible for the reinforcement of the barrier function.